Mouse Clostridium botulinum Neurotoxin Type C1 Heavy Chain Monoclonal Antibody
Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1)
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Clostridium botulinum Neurotoxin Type C1 Heavy Chain; Monoclonal Antibody; Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1); Anti -Clostridium botulinum Neurotoxin Type C1 Heavy Chain (BoNT-C1); anti-Clostridium botulinum Neurotoxin Type C1 Heavy Chain antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11n49
Specificity
Recognizes C. botulinum BoNT-C1 Heavy Chain. In direct ELISA, does not crossreact with the light chains of BoNT-A, -B,-C1,-D, -E, -F, -G, or heavy chains of BoNT-D or -G.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-Clostridium botulinum Neurotoxin Type C1 Heavy Chain antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1ug/ml
Immunoprecipitation: 25ug/ml
Dilution: Western Blot: 1ug/ml
Immunoprecipitation: 25ug/ml
Immunogen
Recombinant protein corresponding to aa866-1291 from C. botulinum BoNT-C1 heavy chain, expressed in E. coli (P18640).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Clostridium botulinum Neurotoxin Type C1 Heavy Chain antibody
BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100kD C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn2+-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291aa, 150kD C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100kD C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50kD N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa866-1291, the C1 heavy chain shares less than 40aa identity with the D-type neurotoxin heavy chain.
Product Categories/Family for anti-Clostridium botulinum Neurotoxin Type C1 Heavy Chain antibody
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Product Notes
The Clostridium botulinum Neurotoxin Type C1 Heavy Chain (Catalog #AAA6000269) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Clostridium botulinum Neurotoxin Type C1 Heavy Chain can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1ug/ml Immunoprecipitation: 25ug/ml. Researchers should empirically determine the suitability of the Clostridium botulinum Neurotoxin Type C1 Heavy Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clostridium botulinum Neurotoxin Type C1 Heavy Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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