Rat anti-Human, Mouse CRB3 Monoclonal Antibody | anti-CRB3 antibody

CRB3 Rat Monoclonal Antibody (14F9)

Cat. Num. AAA588104

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: CRB3; Monoclonal Antibody; CRB3 Rat Monoclonal Antibody (14F9); Crumbs homologue 3; anti-CRB3 antibody

• Host: Rat
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: lgG2c
• Clone Number: 14F9
• Specificity: The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.
• Form/Format: Lyophilized protein G purified in PBS pH7.4
• Sequence Length: 123

• Applicable Applications for anti-CRB3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB 1:1000; IHC: 1:100; IF: 1:100; ; Optimal dilution has to be determined by the user.
• Immunogen: Full length protein
• Target: CRB3
• Preservative: None
• Preparation and Storage: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze·thaw cycles, reconstituted antibodies should be aliquoted before freezing for long·term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.

Western Blot (WB)

(LEFT: Western blot analysis shows that antibody recognize the lull length CRB3 (GST-CRB3) but not the extracellular domain of CRBJ (GST-eRB3extra) (anti-CRB3 (14F9) was used at 1:1000) RIGHT: Western blot analysis shows that antibody recognize overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). (antiCRB3 (14F9) was used at 1:1000).)

Immunohistochemistry (IHC)

(TOP: Immunofluorescence analysis of over expressed human CRB3 In Iysates of HeLa cells. Bottom: Immunofluorescence analysis of endogenous CRB3 expression in the mouse Intestine. OAPI was used to stain the nuclei In HeLa cells. where as E-cadherin staining was used to highlight the lateral membrane in mouse intestine.)

Related Product Information for anti-CRB3 antibody

CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.

References

1. Loie E-CRB3A Controls the Morphology and Cohesion of Cancer Cells through Ehm2/p114RhoGEF-Dependent Signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 37182490
• NCBI GeneID: 92359
• NCBI Official Full Name: CRB3
• NCBI Official Synonym Full Names: crumbs cell polarity complex component 3
• NCBI Official Symbol: CRB3
• NCBI Protein Information: protein crumbs homolog 3
• UniProt Protein Name: Protein crumbs homolog 3
• Protein Family: Protein crumbs
• UniProt Gene Name: CRB3

NCBI Description

This gene encodes a member of the Crumbs family of proteins. This gene is widely expressed in epithelial tissues where the encoded protein isoforms play various roles such as the control of cytokinesis and ciliogenesis or the formation of tight junctions. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Description

Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions.