Mouse anti-Rat MCL Monoclonal Antibody | anti-MCL antibody
MCL, Rat, mAb WEN42
Reactivity
Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
MCL; Monoclonal Antibody; Rat; mAb WEN42; Marcophage C-type lectin; CLR; CLECS89; CLEC4D; anti-MCL antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
WEN42
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-MCL antibody
Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS), Functional Studies (FS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Immunohistochemistry (IHC) Formalin: Frozen splenic sections were fixed in acetone and incubated with WEN42. MHC class I was used as an isotype control.
The typical starting working dilution is 1:50.
The typical starting working dilution is 1:50.
Immunogen
BWZ.36 cell expressing chimeric MCL
Positive Control
Cells of myeloid origin, MCL-transfected 293 cells or CHO cells.
Preparation and Storage
Store at 4 degree C. Under recommended storage conditions, product is stable for at least one year.
Related Product Information for anti-MCL antibody
The monoclonal antibody WEN42 recognizes rat macrophage C-type lectin (MCL). C-type lectin receptors (CLRs) have recently been identified as a fourth family of pattern-recognition receptors for pathogen associated molecular patterns, following TLRs, Nod-like receptors and RIG-I-like receptors. MCL is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC).The complex consists of seven related C-type lectin receptor genes, namely, Dendritic Cell Activating Receptor (DCAR), Dendritic Cell Inhibitory Receptor 1, 2, 3 and 24 (DCIR), Macrophage C-type lectin (MCL) and Macrophage inducible C-type lectin (Mincle). MCL is a type II transmembrane protein with a single extracellular C-terminal C-type lectin-like domain. C-type lectins are functionally diverse. Human MCL is expressed on macrophages (synovial, peritoneal, and blood monocyte-derived), Langerhans cells and on neutrophils, monocytes and immature and mature DCs. In rats, expression of MCL has been detected on macrophages, neutrophils, DCs, B cells, and T cells. Their presence on the surface of immune cells and their potential for recognizing polysaccharide structures suggests a central role as pattern-recognition receptors (PRR) in the innate immune system. Despite the growing amount of data describing expression and function of the APLEC receptors, very little has been reported about MCL in general and the rat MCL in particular. Studies investigating the role of MCL has demonstrated that MCL is capable of inducing phagocytosis, cytokine production and oxidative burst, suggesting an activating role for this protein in the immune response. WEN42 does not bind to cells transfected with human MCL or with other APLEC receptors.
References
1. Lobato-Pascual, A et al; Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells. PLoS ONE 2013, 8: e57406 2. Lobato-Pascual, A et al; Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcepsilonRI-gamma. Eur J Immunol 2013, 43: 3167
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Product Notes
The MCL (Catalog #AAA584777) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MCL, Rat, mAb WEN42 reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCL can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS), Functional Studies (FS), Immunoprecipitation (IP), Western Blot (WB). Immunohistochemistry (IHC) Formalin: Frozen splenic sections were fixed in acetone and incubated with WEN42. MHC class I was used as an isotype control. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the MCL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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