C5a elisa kit
C5a, Human, ELISA Kit
Reactivity
Reactivity: Human. Cross reactivity: Mouse (Weak), Pig (Weak), Rabbit (Weak), Rat (Weak)
Synonyms
C5a; Human; ELISA Kit; C5a elisa kit
Reactivity
Reactivity: Human. Cross reactivity: Mouse (Weak), Pig (Weak), Rabbit (Weak), Rat (Weak)
Application Notes
Application Notes: The human C5a ELISA Kit is to be used for the in vitro quantitative determination of human C5a/C5a-desArg in serum, plasma, sputum and Cerebrospinal fluid (CSF).
Detection Level
0.3 ng/ml
Linearity
The linearity of the assay was determined by serially diluting a sample containing 331 ng/ml human C5a. The diluted samples were measured in the assay. The line obtained a slope of 1.0907 and a correlation coefficient of 0.9988.
Recovery
Plasma
Standard Range
0.3 to 20 ng/ml
Working Volume
100 ul/well
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for C5a elisa kit
Product Description: The complement system is an important factor in innate immunity. During complement activation of complement protein 5 (C5), C5 is proteolytically cleaved and the anaphylatoxic peptide C5a is generated.
C5a is a small polypeptide consisting of 74 aminoacids (11kDa) and is derived from the N-terminus of the alpha chain. C5a itself is very short-lived and in serum is cleaved rapidly into the more stable, though biologically still active C5a-desArg (also called acylation stimulating protein, ASP. Therefore, measurement of C5a-desArg allows reliable conclusions about the level of complement activation in the test samples. For convenience, both forms will be referred to in the following text as C5a.
C5a acts as a potent anaphylatoxin causing smooth muscle contraction, vasodilation, increased vascular permeability, basophil and mast cell degranulation and lysosomal enzyme release. In addition, C5a is a potent chemotactic factor for eosinophils, basophils and monocytes.
C5a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. Elevation of C5a is associated with increased cardiovascular risk in patients with advanced atherosclerosis. Also, C5a is closely associated with the capillary leak syndrome in leukemic children after bone marrow transplantation. C5a is also a marker in urine for predicting the onset of acute graft rejection after kidney transplantation.
Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The human C5a ELISA Kit is based on a catching monoclonal antibody that recognizes a neo-epitope on C5a-desArg. This prevents cross-reactivity with C5
Principle of the assay: The human C5a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
C5a is a small polypeptide consisting of 74 aminoacids (11kDa) and is derived from the N-terminus of the alpha chain. C5a itself is very short-lived and in serum is cleaved rapidly into the more stable, though biologically still active C5a-desArg (also called acylation stimulating protein, ASP. Therefore, measurement of C5a-desArg allows reliable conclusions about the level of complement activation in the test samples. For convenience, both forms will be referred to in the following text as C5a.
C5a acts as a potent anaphylatoxin causing smooth muscle contraction, vasodilation, increased vascular permeability, basophil and mast cell degranulation and lysosomal enzyme release. In addition, C5a is a potent chemotactic factor for eosinophils, basophils and monocytes.
C5a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. Elevation of C5a is associated with increased cardiovascular risk in patients with advanced atherosclerosis. Also, C5a is closely associated with the capillary leak syndrome in leukemic children after bone marrow transplantation. C5a is also a marker in urine for predicting the onset of acute graft rejection after kidney transplantation.
Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The human C5a ELISA Kit is based on a catching monoclonal antibody that recognizes a neo-epitope on C5a-desArg. This prevents cross-reactivity with C5
Principle of the assay: The human C5a ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
References
1. Hartmann H et al; Rapid quantification of C3a and C5a using a combination of chromatographic and immunoassay procedures. J Immunol Meth 1993, 166: 35 2. Stove S et al: Circulating complement proteins in patients with sepsis or systemic inflammatory response syndrome. Clin Diag Lab Immunol 1996, 3:175
3. Krug N et al; Complement factors C3a and C5a are increased in bronchoalveolar lavage fluid after segmental allergen provocation in subjects with asthma. Am J Respir Crit Care Med 2001, 164:1841
3. Krug N et al; Complement factors C3a and C5a are increased in bronchoalveolar lavage fluid after segmental allergen provocation in subjects with asthma. Am J Respir Crit Care Med 2001, 164:1841
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Product Notes
The Reactivity: Human. Cross reactivity: Mouse (Weak), Pig (Weak), Rabbit (Weak), Rat (Weak) C5a (Catalog #AAA584578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA584578 ELISA Kit recognizes Reactivity: Human. Cross reactivity: Mouse (Weak), Pig (Weak), Rabbit (Weak), Rat (Weak) C5a. Application Notes: The human C5a ELISA Kit is to be used for the in vitro quantitative determination of human C5a/C5a-desArg in serum, plasma, sputum and Cerebrospinal fluid (CSF). Researchers should empirically determine the suitability of the C5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C5a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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