Mouse anti-Mouse MPO Monoclonal Antibody | anti-MPO antibody

MPO, Mouse, mAb 8F4, FITC

Cat. Num. AAA584384

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: MPO; Monoclonal Antibody; Mouse; mAb 8F4; FITC; anti-MPO antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
• Sequence Length: 745

• Applicable Applications for anti-MPO antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA)
• Application Notes: Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Conjugate: FITC
• Immunogen: Purified mouse MPO from WEHI-3 cells
• Negative Control: Lymphocytes isolated from digested infarcts
• Positive Control: Neutrophils isolated from digested infarcts
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-MPO antibody

Product Description: The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa.- MPO is- stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms.
Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an- increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient- mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.
The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA,- in frozen tissue sections fixed with acetone and in flow cytometry using a- cell permeabilization method. The antibody 8F4 is cross-reactive- with rat MPO

References

1. Huugen, D et al; Aggravation of anti-myeloperoxidase antibody-induced glomerulonephritis by bacterial lipopolysaccharide: role of tumor necrosis factor-alpha. Am J Pathol 2005, 167: 47 2. Wang, D et al; C/EBPalpha directs monocytic commitment of primary myeloid progenitors. Blood 2006, 108:1223
3. Matthijsen, M et al; Myeloperoxidase is critically involved in the induction of organ damage after renal ischemia reperfusion. Am J Pathol 2007, 171: 1743
4. Leeuwen van, M et al; Accumulation of myeloperoxidase-positive neutrophils in atherosclerotic lesions in LDLR -/- mice. Arterioscler Thromb Vasc Biol 2008, 28: 84
5. Nahrendorf, M et al; An activatable MR imaging agent reports myeloperoxidase activity in healing infarcts and detects the anti-inflammatory effects of atorvastatin on ischemia-reperfusion injury non-invasively. Circulation 2008, 117: 1153

NCBI and Uniprot Product Information

• NCBI GI #: 4557759
• NCBI GeneID: 4353
• NCBI Accession #: NP_000241.1
• NCBI GenBank Nucleotide #: NM_000250.1
• Molecular Weight: 87,248 Da
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• Protein Family: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN

NCBI Description

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

Uniprot Description

MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; response to mechanical stimulus; hydrogen peroxide catabolic process; response to yeast; response to lipopolysaccharide; defense response; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease