anti-Mouse MBL-A Monoclonal Antibody | anti-MBL-A antibody

MBL-A, Mouse, mAb 2B4

Cat. Num. AAA584309

• Gene Names: Mbl1; MBL-A; MBP-A; S-MBP
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: MBL-A; Monoclonal Antibody; Mouse; mAb 2B4; anti-MBL-A antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 239

• Applicable Applications for anti-MBL-A antibody: Immunoassay (IA), Western Blot (WB)
• Application Notes: Application Notes: The monoclonal antibody 2B4 can be used for immuno assay and Western blotting (non-reduced). The antibody 2B4 is not useful for immunohistology.; Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (2B4 is a calcium-dependent antibody).
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-MBL-A antibody

Product Description: Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 2B4 is a calcium-dependent antibody.

References

1. Liu, H et al; Characterization and Quantification of Mouse Mannan-Binding Lectins (MBL-A and MBL-C) and Study of Acute Phase Responses. Scand J Immunol 2001, 53: 489 2. Sastry, K et al; Molecular characterization of the mouse mannose-binding proteins. The mannose-binding protein A but not C is an acute phase reactant. J Immunol 1991, 147: 692

NCBI and Uniprot Product Information

• NCBI GI #: 6754654
• NCBI GeneID: 17194
• NCBI Accession #: NP_034905.1
• NCBI GenBank Nucleotide #: NM_010775.2
• Molecular Weight: 15,600 Da
• NCBI Official Full Name: mannose-binding protein A
• NCBI Official Synonym Full Names: mannose-binding lectin (protein A) 1
• NCBI Official Symbol: Mbl1
• NCBI Official Synonym Symbols: MBL-A; MBP-A; S-MBP
• NCBI Protein Information: mannose-binding protein A; raRF p28B; mannan-binding protein; mannose binding lectin (A); mannose binding lectin, serum (A); ra-reactive factor polysaccharide-binding component p28B
• UniProt Protein Name: Mannose-binding protein A
• UniProt Gene Name: Mbl1
• UniProt Synonym Gene Names: MBP-A; RaRF p28B
• UniProt Entry Name: MBL1_MOUSE

Uniprot Description

Mbl1: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: extracellular space; collagen; extracellular region

Molecular Function: mannose binding; protein homodimerization activity; protease binding; calcium ion binding; carbohydrate binding; calcium-dependent protein binding; polysaccharide binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; immune system process; positive regulation of phagocytosis; innate immune response; complement activation, lectin pathway; complement activation, classical pathway; protein homotetramerization