Mouse Mannose Binding Lectin (MBL) ELISA Kit | MBL elisa kit

Mouse Mannose Binding Lectin (MBL) ELISA Kit

Cat. Num. AAA454844

• Gene Names: Mbl1; MBL-A; MBP-A; S-MBP
• Reactivity: Mouse
• Synonyms: Mannose Binding Lectin (MBL); Mouse Mannose Binding Lectin (MBL) ELISA Kit; MBL2 COLEC1 HSMBPC MBP1 MBP Collectin-1 Mannose-binding protein C Mannan Binding Protein Mannose-Binding Lectin(Protein C)2; Soluble(Opsonic Defect); MBL elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MBL. No significant cross-reactivity or interference between MBL and analogues was observed.
• Sequence Length: 239

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MBL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MBL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBL elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MBL in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MBL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MBL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6754654
• NCBI GeneID: 17194
• NCBI Accession #: NP_034905.1
• NCBI GenBank Nucleotide #: NM_010775.2
• UniProt Accession #: P39039; Q0P6H1
• Molecular Weight: 15,600 Da
• NCBI Official Full Name: mannose-binding protein A
• NCBI Official Synonym Full Names: mannose-binding lectin (protein A) 1
• NCBI Official Symbol: Mbl1
• NCBI Official Synonym Symbols: MBL-A; MBP-A; S-MBP
• NCBI Protein Information: mannose-binding protein A
• UniProt Protein Name: Mannose-binding protein A
• Protein Family: MreB-like protein
• UniProt Gene Name: Mbl1
• UniProt Synonym Gene Names: MBP-A; RaRF p28B

Uniprot Description

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages ().