Rat anti-Mouse M-CSF Monoclonal Antibody | anti-M-CSF antibody
M-CSF, Mouse, mAb ER-MP58
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
M-CSF; Monoclonal Antibody; Mouse; mAb ER-MP58; anti-M-CSF antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Sequence Length
153
Applicable Applications for anti-M-CSF antibody
F, Flow Cytometry (FC/FACS)
Application Notes
Application Use: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-M-CSF antibody
Product Description: The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells ut to the monocytic level, but also on grnaulocytes. ERxpression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commenly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hemopoietic islands in various organs, and for embryonal studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 (HM1084) and ER-MP-20 (HM1082).
References
1. Cook, A et al; The effect of tissue type-plasminogen activator deletion and associated fibrin(ogen) deposition on macrophage localization in peritoneal inflammation. Thromb Haemost 2006, 95: 659
2. Sunderk¶tter, C et al; Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J Immunol 2004, 172: 4410
3. Nikolic, T et al; Developmental stages of myeloid dendritic cells in mouse bone marrow. Int Immunol 2004, 15: 515
4. Chan, J et al; Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression. Blood 1998, 92: 1423
5. De Bruijn, M et al; High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage. Eur J Immunol 1996, 26: 2850
6. Leenen, P et al; Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens. Eur J Immunol 1990, 20: 27
2. Sunderk¶tter, C et al; Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J Immunol 2004, 172: 4410
3. Nikolic, T et al; Developmental stages of myeloid dendritic cells in mouse bone marrow. Int Immunol 2004, 15: 515
4. Chan, J et al; Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression. Blood 1998, 92: 1423
5. De Bruijn, M et al; High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage. Eur J Immunol 1996, 26: 2850
6. Leenen, P et al; Murine macrophage precursor characterization II. Monoclonal antibodies against macrophage precursor antigens. Eur J Immunol 1990, 20: 27
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
macrophage colony-stimulating factor, M-CSF {N-terminal}, partial
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Product Notes
The M-CSF (Catalog #AAA584294) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The M-CSF, Mouse, mAb ER-MP58 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's M-CSF can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS). Application Use: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the M-CSF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M-CSF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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