Rabbit anti-Human RNA Polymerases I and III Subunit AC1 Polyclonal Antibody | anti-POLR1C antibody

RNA Polymerases I and III Subunit AC1, CT (DNA-directed RNA Polymerases I And III Subunit RPAC1, POLR1C, DNA-directed RNA Polymerase I Subunit C, DNA-directed RNA Polymerases I And III 40kD Polypeptide, RPA40, RPC40, AC40, RPA39, POLR1E) (MaxLight 550)

Cat. Num. AAA6498067

• Gene Names: POLR1C; AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: RNA Polymerases I and III Subunit AC1; Polyclonal Antibody; CT (DNA-directed RNA Polymerases I And III Subunit RPAC1; POLR1C; DNA-directed RNA Polymerase I Subunit C; DNA-directed RNA Polymerases I And III 40kD Polypeptide; RPA40; RPC40; AC40; RPA39; POLR1E) (MaxLight 550); anti-POLR1C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human POLR1C.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-POLR1C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human POLR1C (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-POLR1C Antibody (C-term) in A2058 cell line lysates (35ug/lane). POLR1C (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with POLR1C antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-POLR1C antibody

Antibodies; Enzymes; Polymerase

References

1. Rush, J., Nat. Biotechnol. 23(1), 94-101 (2005). 2. Hirschler-Laszkiewicz, I., J. Biol. Chem. 278(21), 18953-18959 (2003). 3. Dammann, R., Biochim. Biophys. Acta 1396(2), 153-157 (1998). 4. Seither, P., Chromosoma 106(4), 216-225 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 42560246
• NCBI GeneID: 9533
• NCBI Accession #: NP_976035
• NCBI GenBank Nucleotide #: NM_203290.3
• UniProt Accession #: O15160; O75395; Q5JTE3
• Molecular Weight: 41.6 kDa (369aa) confirmed by MALDI-TOF
• NCBI Official Full Name: DNA-directed RNA polymerases I and III subunit RPAC1 isoform 1
• NCBI Official Synonym Full Names: RNA polymerase I subunit C
• NCBI Official Symbol: POLR1C
• NCBI Official Synonym Symbols: AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
• NCBI Protein Information: DNA-directed RNA polymerases I and III subunit RPAC1
• UniProt Protein Name: DNA-directed RNA polymerases I and III subunit RPAC1
• Protein Family: DNA-directed RNA polymerases
• UniProt Gene Name: POLR1C
• UniProt Synonym Gene Names: POLR1E; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA40
• UniProt Entry Name: RPAC1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 2.7.7.6; Transferase

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol; DNA-directed RNA polymerase I complex

Molecular Function: protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; negative regulation of gene expression, epigenetic; positive regulation of interferon type I production; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; innate immune response; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase III promoter

Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3