Rat anti-Mouse TREM-1 Monoclonal Antibody | anti-TREM-1 antibody

TREM-1, Mouse, mAb L5-B8

Cat. Num. AAA584278

• Gene Names: TREM1; CD354; TREM-1
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: TREM-1; Monoclonal Antibody; Mouse; mAb L5-B8; triggering receptor expressed on myeloid cells; TREM1; anti-TREM-1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 225

• Applicable Applications for anti-TREM-1 antibody: Flow Cytomery(FC/FACS), FS, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
• Immunogen: Mouse TREM-1-human IgG-Fc fusion protein
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TREM-1 antibody

Product Description: The monoclonal antibody L5-B8 recognizes-mouse triggering receptor expressed on myeloid cells-1 (TREM-1). TREM-1 is a 30 kDa monomeric transmembrane activating receptor. TREM-1 is a member of the immunoglobulin superfamily. TREM-1 is expressed at low levels in the early development of the hematopoietic system in the promonoistic stage, and at high levels on the surface of immune cells, including neutrophils, monocytes and macrofages. TREM-1 is synthesized as a 234 amino acid (aa) precursor with a signal peptide (16 aa), an extracellular domain (184 aa), a transmembrane domain (29 aa), and a short cytoplasmic domain (5 aa). The short intracellular domain associates with a signal-transduction molecule, DNAX-activation protein 12 (DAP12), triggering the secretion of inflammatory cytokines that amplify the host response to microbial agents. TREM-1 acts in synergy with Toll-like receptor signaling pathways in amplifying the inflammatory response. Platelets express a natural ligand for TREM-1.
The expression of TREM-1 is greatly upregulated on phagocytic cells in the presence of bacteria and fungi. TREM-1 has a role in sepsis, inflammatory bowel disease (IBD) and multiple sclerosis. In contrast, TREM-1 is not upregulated in samples from patients with non-inefctious inflammatory conditions. During infections, receptor expression is modulated and soluble TREM-1 (sTREM-1, 17 kDa) is released. TREM-1 is shed from the membrane of activated phagocytes and can be found as sTREM-<1 in> body fluids like plasma and bronchoalveolar lavage fluid (BAL).
Elavated levels of sTREM-1 have a accuracy and sensitivity in detecting microbial infections as underlying disease. Furthermore, sTREM-1 has been associated with non-infectious inflammatory conditions like major abdominal surgery, peptic ulcer disease and COPD.

NCBI and Uniprot Product Information

• NCBI GI #: 336088680
• NCBI GeneID: 54210
• NCBI Accession #: NP_001229518.1
• NCBI GenBank Nucleotide #: NM_001242589.1
• Molecular Weight: 25,574 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 1 isoform 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: TREM1
• NCBI Official Synonym Symbols: CD354; TREM-1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• UniProt Gene Name: TREM1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_HUMAN

NCBI Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; plasma membrane; integral to membrane

Molecular Function: receptor activity

Biological Process: neutrophil chemotaxis; innate immune response; cytokine secretion during immune response; blood coagulation; chemokine metabolic process; leukocyte migration; humoral immune response