Mouse C1qBP Monoclonal Antibody | anti-C1qBP antibody

C1qBP, Human, mAb 74.5.2

Cat. Num. AAA584062

• Gene Names: C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
• Reactivity: Reactivity: Human. Cross reactivity: Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: C1qBP; Monoclonal Antibody; Human; mAb 74.5.2; Glycoprotein gC1qBP; GC1q-R protein; hyaluronan-binding protein 1; mitochondrial matrix protein p32; p33; anti-C1qBP antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 282

• Applicable Applications for anti-C1qBP antibody: Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Westen Blot (WB)
• Application Notes: Application Use: For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Immunogen: Recombinant-gC1qR corresponding to maturegC1qR (amino acids 74-282)
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-C1qBP antibody

Product Description: The monoclonal antibody 74.5.2 recognizes a cell membrane C1q binding molecule that recognises the globular heads of C1q. It is also present in plasma and the extracellular matrix. The molecule is an unusually acidic, single chain protein with an apparent molecular weight of 33 kDa. It does not possess a conventional sequence motif compatible with a membrane spanning segment nor a consensus site for a GPI anchor. gC1qR migrates as a single chain under both reducing and non-reducing conditions, but it behaves as an oligomer on gel-filtration in non-dissociating conditions. Its multimer formation may be a critical process by which the gC1qR molecule increases its affinity for multivalent ligands such as C1q. gC1qR has been shown to inhibit complement activation by preventing the binding of C1q to antibodies, suggesting that the binding site for gC1qR and the binding site for immune complexes, which are present on the C1q globular ˜heads, may be located at the same position. gC1qR is capable of interacting with several proteins involved in blood clotting, namely, thrombin, prothrombin, the heparinbinding form of vitronectin, the ternary complex, vitronectin-thrombin-antithrombin, as well as high-molecular-weight kininogen and Hageman factor. Besides its role in the complement pathway, gC1qR participates in enhancement of Fc-receptor and CR1-mediated phagocytosis, procoagulant activity on platelets, and chemotactic activity on mast cells, eosinophils, neutrophils, and fibroblasts. gC1qR is expressed on a wide variety of cells and can serve as a binding site for plasma and microbial proteins. Its antigenic sites may be cryptic on cells in suspension but become exposed when the cells are fixed by bifunctional cross-linkers. Probably it is also expressed on the cell membrane as a tetramer. Crosslinking or activation may thus bring about a tetrameric assembly of gC1qR followed by a conformational change within the molecule, thereby exposing epitopes which are otherwise hidden. A form of gC1qR is also found inside the cell. Intracellular gC1qR has been shown to bind the cytoplasmic tail of the alpha1B-adrenergic receptor and to PKC u.
The monoclonal antibody 74.5.2 is directed against epitopes in the XC15 peptide that contains a binding site for high-molecular-weight kininogen and Factor XII. Clone 74.5.2 recognizes both the mature (74 - 282) and truncated form, lacking residues 74-95.

References

1. Ghebrehiwet, B et al; Identification of functional domains on gC1Q-R, a cell surface protein that binds to the globular "heads" of C1Q, using monoclonal antibodies and synthetic peptides. Hybridoma 1996, 5: 333
2. Ghebrehiwet, B et al; gC1q-R/p33, a member of a new class of multifunctional and multicompartmental cellular proteins, is involved in inflammation and infection. Immunol Rev 2001, 180: 65
3. Peerschke, E et al; gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis. Infect Immun 2006, 74: 4418
4. Sansonno, D et al; Role of the receptor for the globular domain of C1q protein in the pathogenesis of hepatitis C virau-related cryoglobulin vascular damage. J Immunol 2009, 183: 6013

NCBI and Uniprot Product Information

• NCBI GI #: 4502491
• NCBI GeneID: 708
• NCBI Accession #: NP_001203.1
• NCBI GenBank Nucleotide #: NM_001212.3
• Molecular Weight: 31,362 Da
• NCBI Official Full Name: complement component 1 Q subcomponent-binding protein, mitochondrial
• NCBI Official Synonym Full Names: complement component 1, q subcomponent binding protein
• NCBI Official Symbol: C1QBP
• NCBI Official Synonym Symbols: p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
• NCBI Protein Information: complement component 1 Q subcomponent-binding protein, mitochondrial; p33; glycoprotein gC1qBP; hyaluronan-binding protein 1; ASF/SF2-associated protein p32; mitochondrial matrix protein p32; C1q globular domain-binding protein; splicing factor SF2-associated protein
• UniProt Protein Name: Complement component 1 Q subcomponent-binding protein, mitochondrial
• Protein Family: Complement component 1 Q subcomponent-binding protein
• UniProt Gene Name: C1QBP
• UniProt Synonym Gene Names: GC1QBP; HABP1; SF2P32; C1qBP
• UniProt Entry Name: C1QBP_HUMAN

NCBI Description

The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein. [provided by RefSeq, Jul 2008]

Uniprot Description

C1QBP: a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. Originally identified via its binding interactions with Splicing Factor (SF-2). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases, the tumor suppressor ARF, and multiple antigens of bacterial and viral origin. Overexpressed in a number of cancer cell types, and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis. Inhibits the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress. Binding to C1q inhibits C1. In complex with cytokeratin-1/KRT1 is a high affinty receptor for kininogen-1/HMWK. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. The cell surface form may require docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. Belongs to the MAM33 family.

Protein type: Nucleolus; Mitochondrial; Receptor, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extracellular space; cell surface; mitochondrion; membrane; mitochondrial matrix; cytoplasm; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; adrenergic receptor binding; protein kinase C binding; complement component C1q binding; hyaluronic acid binding; kininogen binding; transcription factor binding; transcription corepressor activity

Biological Process: phosphoinositide 3-kinase cascade; negative regulation of interleukin-12 production; positive regulation of cell adhesion; transcription, DNA-dependent; viral reproduction; apoptosis; positive regulation of apoptosis; RNA splicing; negative regulation of defense response to virus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein kinase B signaling cascade; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of interferon-gamma production; regulation of complement activation; innate immune response; immune response; mRNA processing; blood coagulation; complement activation, classical pathway; blood coagulation, intrinsic pathway; mature ribosome assembly