Mouse TLR2 Monoclonal Antibody | anti-TLR2 antibody

TLR2, Human, mAb TL2.1, FITC

Cat. Num. AAA584056

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Monkey
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: TLR2; Monoclonal Antibody; Human; mAb TL2.1; FITC; CD282; anti-TLR2 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Monkey
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: F, Flow Cytometry (FC/FACS), P, Western Blot (WB
• Application Notes: Application Notes: FC: 2 ug antibody per 300000 cells; untreated as well as 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells or use 4 % faraformaldehyde as fixative! (Ref 5); Application Use: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
• Conjugate: FITC
• Immunogen: Recombinant human TLR2 produced by the CHO/CD14 reporter cell line
• Positive Control: Peripheral blood mononuclear cells, granulocytes and monocytes.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label

Related Product Information for anti-TLR2 antibody

Product Description: The monoclonal antibody TL2.1 recognizes human Toll-like receptor 2 (TLR2, CD282). Toll-like receptors (TLR) are highly conserved throughout evolution and are involved in the innate defence to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs are identified as type I transmembrane signaling receptors with pattern recognition capabilities. They have been implicated in the innate host defence to pathogens. TLR2 is expressed on macrophages, smooth muscle, lung, spleen, thymus, brain and adipose tissue. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. TLR2 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It cooperates with TLR1 to mediate te innate immune response to bacterial lipoproteins or lipopeptides. It acts via MYD88 and TRAF6, leading to NF- degree -B activation, cytokine secretion and the inflammatory response. TLR2 also promotes apoptosis in response to lipoproteins. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, S. aureus, B Burgdorferi, T pallidum, M fermentans and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2. The monoclonal antibody TL2.1 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells.

References

1. Lien, E et al; Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem 1999, 274: 33419 2. Flo, T et al; Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 2001, 69: 474
3. Faure, E et al; Bacterial lipopolysaccharide and IFN-γ induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-κB activation. J Immunol 2001, 166: 2018
4. Droemann, D et al; Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung. Histochem Cell Biol 2003, 119: 103
5. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow- cytometry. Vet Immunol Immunopathol 2008, 124; 184

NCBI and Uniprot Product Information

• NCBI GI #: 19718734
• NCBI GeneID: 7097
• NCBI Accession #: NP_003255.2
• NCBI GenBank Nucleotide #: NM_003264.3
• Molecular Weight: 89,838 Da
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Membrane protein, integral; Apoptosis; Cell surface; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell surface; cell projection; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; triacylated lipoprotein binding; protein heterodimerization activity; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of leukocyte migration; response to toxin; leukotriene metabolic process; positive regulation of NF-kappaB import into nucleus; detection of triacylated bacterial lipoprotein; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; toll-like receptor 4 signaling pathway; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; defense response to Gram-positive bacterium; myelin formation in the central nervous system; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; apoptosis; positive regulation of interleukin-12 production; microglial cell activation; response to molecule of fungal origin; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of toll-like receptor signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; negative regulation of interleukin-17 production; response to hypoxia; toll-like receptor signaling pathway; innate immune response; immune response; chloramphenicol transport; positive regulation of inflammatory response; positive regulation of cytokine secretion

Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To