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Mouse IgY, Chicken, mAb 7C2 Monoclonal Antibody

IgY, Chicken, mAb 7C2

Applications
Immunoprecipitation, Western Blot
Synonyms
IgY; Chicken; mAb 7C2; Monoclonal Antibody; anti-IgY; mAb 7C2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Reactivity: N/A. Cross reactivity: Duck
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-IgY, Chicken, mAb 7C2 antibody
Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-IgY, Chicken, mAb 7C2 antibody
Product Description: The monoclonal antibody 7C2 can be used during various purification steps of IgY. The yolk of eggs laid by immunized chickens has been recognized as an excellent source of polyclonal antibodies (pAb). Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. Because a single egg contains as much antibody as an average bleed from a rabbit, this simple, non-invasive approach presents an appealing alternative to conventional pAb production methods. Purification of chicken egg yolk immunoglobulin Y (IgY), the 150 kDa IgG homolog, does not require animal bleeding. In addition, the eggs from immunized chickens provide a continual, daily source of pAb, and this convenient approach offers greater compatibility with animal protection regulations. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens. Highly conserved mammalian proteins sometimes fail to illicit a humoral response in animals, such as rabbits, that are traditionally used for generating pAb. Non-specific binding and need for cross-species immunoabsorptions is eliminated since chicken IgY does not cross-react with mammalian IgG and does not bind bacterial or mammalian Fc receptors. There are well defined structural differences of IgY-type immunoglobulins and the IgG of mammals. That includes the molar mass of the heavy chains of the immunoglobulins. The IgY-type immunoglobulins are much less flexible than IgG. Also, the structures of the Fc part of the immunoglobulin isotypes IgY and IgG are different. The 7C2 antibody is cross reactive for duck

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Product Notes

The IgY, Chicken, mAb 7C2 (Catalog #AAA584038) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgY, Chicken, mAb 7C2 reacts with Reactivity: N/A. Cross reactivity: Duck and may cross-react with other species as described in the data sheet. AAA Biotech's IgY, Chicken, mAb 7C2 can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB). Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Researchers should empirically determine the suitability of the IgY, Chicken, mAb 7C2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgY, Chicken, mAb 7C2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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