Mouse BPDE-DNA Monoclonal Antibody | anti-BPDE-DNA antibody

BPDE-DNA, mAb 5D11

Cat. Num. AAA584032

• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
• Synonyms: BPDE-DNA; Monoclonal Antibody; mAb 5D11; anti-BPDE-DNA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-BPDE-DNA antibody: Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), P
• Application Notes: Application Notes: FC: Washed sperm was fixed in 2% paraformaldehyde and permeabilized with 0.2% triton x-100/0.1%; Application Use: For immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
• Immunogen: BPDE-I-DNA complexed with methylated BSA
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-BPDE-DNA antibody

Product Description: A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic. The monoclonal antibody 5D11 recognizes BPDE-I-DNA (PAH-DNA).

References

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