Rat anti-Mouse Mclass II Monoclonal Antibody

Mclass II, Mouse, mAb ER-TR3

Cat. Num. AAA584015

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: Mclass II; Monoclonal Antibody; Mouse; mAb ER-TR3; anti-Mclass II antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-Mclass II antibody: F, Flow Cytometry (FC/FACS)
• Application Notes: Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-Mclass II antibody

Product Description: The monoclonal antibody ER-TR3 reacts with mouse major histocompatibitity complex class II (MHC class II), also known as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II. The monoclonal antibody ER-TR3 is a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells and macrophages) and for studying the development of T helper cells since they stain stromal cells in thymus. The level of antigen detected by ER-TR3 differs from strain to strain. The monoclonal antibody ER-TR3 cross reacts with human MHC class II on tonsil. Distribution of ER-TR3 among mouse strains with independent and recombinant haplotypes.

References

1. Van Vliet, E et al; Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 1984, 14: 524 2. Van Vliet, E et al; Stromal cell types in the developing thymus of the normal and nude mouse embryo. Eur J Immunol 1985, 15: 675