Rabbit C3c Polyclonal Antibody | anti-c3c antibody

Rabbit anti Human C3c

Cat. Num. AAA573254

• Gene Names: c3c; C3-3; c3-2; unp301; zgc:103610
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
• Applications: Immunoprecipitation
• Synonyms: C3c; Polyclonal Antibody; Rabbit anti Human C3c; anti-c3c antibody

• Host: Rabbit
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
• Clonality: Polyclonal
• Specificity: Precipitating polyclonal Rabbit antiSerum to C3c subunit of Human complement C3.

• Applicable Applications for anti-c3c antibody: Immunoprecipitation (IP)
• Application Notes: Precipitation assays. In immunoelectrophoresis use 2 ul human plasma or equivalent against 120 ul antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
• Product Type: Primary Antibodies
• Source: C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Product: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
• Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
• Preparation and Storage: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Related Product Information for anti-c3c antibody

In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3b or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.

Product Categories/Family for anti-c3c antibody

Complement; Coagulation; Haematology

NCBI and Uniprot Product Information

• NCBI GI #: 82524318
• NCBI GeneID: 565074
• NCBI Accession #: NP_001032313.1
• NCBI GenBank Nucleotide #: NM_001037236.1
• NCBI Official Full Name: complement component c3c
• NCBI Official Synonym Full Names: complement component c3c
• NCBI Official Symbol: c3c
• NCBI Official Synonym Symbols: C3-3; c3-2; unp301; zgc:103610
• NCBI Protein Information: complement component c3c; complement component C3-2