Swine anti Human IgG (Fc specific) Secondary Antibody

Swine anti Human IgG (Fc specific)

Cat. Num. AAA571923

• Synonyms: Swine anti Human IgG (Fc specific); Polyclonal Secondary Antibody; Swine anti Human IgG (Fc specific) secondary antibody

• Host: Swine
• Clonality: Polyclonal
• Specificity: The reactivity of the antiserum is restricted to the Fc part of IgG. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments.
• Form/Format: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Concentration: Protein concentration||Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal swine serum. No foreign proteins added. (varies by lot)

• Preservative: No preservative added.
• Immunogen: Highly purified Fc fragment of normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
• Antibody titre: Precipitin titre 1 :64 when tested against pooled normal human serum in agar-block immunodiffusion titration.
• Intended use: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in human serum or other body fluids or to determine its concentration.To prepare an immunoadsorbent for the purification of human IgG from serum or plasma.
• Directions for use: In immunoelectrophoresis use 2 uL serum or equivalent against 120 uL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 uL antiserum in 3 mm diameter center well and 2 uL serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
• Packing: Vial with 1 mllyophilized antiserum.
• Handling: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 degree C, not refrozen, and preferably used the same day.
• Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
• Preparation and Storage: Storage I shelf life:; Lyophilized at +4 degree C = at least 10 years; Reconstituted at or below -20 degree C = 3-5 years; Reconstituted at +4 degree C =7 days

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Precipitating polyclonal swine antiserum to human IgG, Fc specific

Product Categories/Family for Swine anti Human IgG (Fc specific) secondary antibody

Antisera