Cold Shock Domain Containing Protein E1 Recombinant Protein | CSDE1 recombinant protein

Recombinant Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1)

Cat. Num. AAA2030186

• Gene Names: CSDE1; UNR; D1S155E
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cold Shock Domain Containing Protein E1; Recombinant Cold Shock Domain Containing Protein E1; RNA Binding (CSDE1); CSDE1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MSFDPNLLHN NGHNGYPNGT SAALRETGVI EKLLTSYGFI QCSERQARLFFHCSQYNGNL QDLKVGDDVE FEVSSDRRTG KPIAVKLVKI KQEILPEERMNGQVVCAVPH NLESKSPAAP GQSPTGSVCY ERNGEVFYLT YTPEDVEGNVQLETGDKINF VIDNNKHTGA VSARNIMLLK KKQARCQGVV CAMKEAFGFIERGDVVKEIF FHYSEFKGDL ETLQPGDDVE FTIKDRNGKE VATDV
• Sequence Length: 844

• Applicable Applications for CSDE1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.0kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Val245
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 338968930
• NCBI GeneID: 7812
• NCBI Accession #: NP_001229820.1
• NCBI GenBank Nucleotide #: NM_001242891.1
• Molecular Weight: 93,742 Da
• NCBI Official Full Name: cold shock domain-containing protein E1 isoform 4
• NCBI Official Synonym Full Names: cold shock domain containing E1, RNA-binding
• NCBI Official Symbol: CSDE1
• NCBI Official Synonym Symbols: UNR; D1S155E
• NCBI Protein Information: cold shock domain-containing protein E1
• UniProt Protein Name: Cold shock domain-containing protein E1
• Protein Family: Cold shock domain-containing protein
• UniProt Gene Name: CSDE1
• UniProt Synonym Gene Names: D1S155E; KIAA0885; NRU; UNR
• UniProt Entry Name: CSDE1_HUMAN

Uniprot Description

CSDE1: RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA- binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding- region determinant of instability (mCRD) domain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Adaptor/scaffold; RNA-binding

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi apparatus; mitochondrial inner membrane; cytoplasm; plasma membrane

Molecular Function: DNA binding

Biological Process: regulation of transcription, DNA-dependent; male gonad development