Goat anti Human IgM (Fc specific) Secondary Antibody

Goat anti Human IgM (Fc specific)

Cat. Num. AAA571528

• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Dot Blot, Immunoblot, Western Blot
• Synonyms: Goat anti Human IgM (Fc specific); Polyclonal Secondary Antibody; Goat anti Human IgM (Fc specific) secondary antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Specificity Reference Reagent. IgG fraction of polyclonal Goat antiSerum to Human IgM, Fc specific.

• Applicable Applications for Goat anti Human IgM (Fc specific) secondary antibody: Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)
• Application Notes: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Precipitin titre not less than 1:32 when tested against pooled normal human serum in agar-block immunodiffusion titration.
• Source: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Product: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. IgG protein concentration 10 mg/ml. No foreign proteins added.
• Reconstitution: It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
• Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
• Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but MBS accepts no liability for any inaccuracies or omissions in this information.
• Preparation and Storage: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Related Product Information for Goat anti Human IgM (Fc specific) secondary antibody

The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgA and IgG/Fab or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Product Categories/Family for Goat anti Human IgM (Fc specific) secondary antibody

Secondary Antibody