Goat anti Monkey IgG (heavy and light chains) Secondary Antibody

Goat anti Monkey IgG (heavy and light chains)

Cat. Num. AAA571208

• Reactivity: Monkey
• Applications: Immunofluorescence, ELISA, Dot Blot, Immunoblot, Western Blot
• Synonyms: Goat anti Monkey IgG (heavy and light chains); Polyclonal Secondary Antibody; Goat anti Monkey IgG (heavy and light chains) secondary antibody

• Host: Goat
• Reactivity: Monkey
• Clonality: Polyclonal
• Specificity: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
• Form/Format: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS,pH 7.2).

• Applicable Applications for Goat anti Monkey IgG (heavy and light chains) secondary antibody: Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)
• Application Notes: Intended Use: As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.; ; Dilutions: Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
• Preservative: No preservative added, as it may interfere with the antibody activity. No foreign protein added.
• Immunogen: Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
• Adsorption: No adsorption required.
• Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with IgG in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The product may also react with IgG of other species as has been observed for chimpanzee and man.
• Physicochemical characteristics: IgG protein concentration 10 mg/ml. No foreign proteins added.
• Antibody titre: Precipitin titre not less than 1:64 when tested against normal monkey serum in agar block titration.
• Preparation and Storage: Handling: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.; ; Packing: Vial with 10 mg lyophilized purified hyperimmune IgG.; ; Storage / shelf life:; Lyophilized at +4°C at least 10 years; reconstituted at or below -20°C from 3-5 years; reconstituted at +4°C for 7 days

Related Product Information for Goat anti Monkey IgG (heavy and light chains) secondary antibody

Purified IgG fraction of polyclonal goat antiserum to monkey IgG, heavy and light chains

Product Categories/Family for Goat anti Monkey IgG (heavy and light chains) secondary antibody

Secondary Antibody