Mouse EpCAM/CD326/EPG-2/SCLC-CD2 Monoclonal Antibody | anti-EpCAM/CD326/EPG-2/SCLC-CD2 antibody
Mouse anti EpCAM/CD326/EPG-2/SCLC-CD2 epithelial antigen
Gene Names
Epcam; Egp314; Tacstd1
Reactivity
Rat. Does not react with human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Western Blot
Synonyms
EpCAM/CD326/EPG-2/SCLC-CD2; Monoclonal Antibody; Mouse anti EpCAM/CD326/EPG-2/SCLC-CD2 epithelial antigen; anti-EpCAM/CD326/EPG-2/SCLC-CD2 antibody
Host
Mouse
Reactivity
Rat. Does not react with human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GZ-20
Specificity
The antibody GZ-20 is directed against rat EpCAM and recognises an epitope that is distinct from the epitope recognised by the GZ-1 antibody. The GZ-20 antibody does not cross react with human EpCAM.
Form/Format
Each vial contains 100ug 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide.
Sequence Length
315
Applicable Applications for anti-EpCAM/CD326/EPG-2/SCLC-CD2 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
Application Notes
The GZ-20 antibody is suitable for the detection of rat EpCAM by western blotting, immunoprecipitation, immunocytochemistry and flow cytometry.
The antibody is also suitable for the detection of rat EpCAM by immunohistochemistry on frozen (acetone fixation for 10 minutes at -20 degree C) and paraffin embedded tissues.
Optimal antibody dilutions for the different applications should be determined by titration.
The antibody is also suitable for the detection of rat EpCAM by immunohistochemistry on frozen (acetone fixation for 10 minutes at -20 degree C) and paraffin embedded tissues.
Optimal antibody dilutions for the different applications should be determined by titration.
Source Note
GZ-20 is a mouse monoclonal IgG1 antibody derived by fusion of P3-NS1/1-Ag4-1 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with cells derived from the basolateral membrane of rat small intestine.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-EpCAM/CD326/EPG-2/SCLC-CD2 antibody
The epithelial cell adhesion molecule (EpCAM), also known as CD326, is a 40 kD glycoprotein that is expressed at the basolateral cell membrane of simple, pseudo-stratified and transitional epithelia of normal adult tissues. EpCAM is reported to function as a homotypic calcium-independent cell adhesion molecule but studies suggest that EpCAM is also involved in cellular processes such as cell signalling, migration, proliferation and differentiation.
In humans, EpCAM is overexpressed in a wide range of epithelial carcinomas including lung, colorectal, breast, prostate, head and neck, and hepatic carcinomas. The EpCAM molecule has, therefore, been widely used as a marker for human carcinomas and has been considered as a potential target for immunotherapy. EpCAM is also present on exosomes, microvesicles that are abundantly secreted by tumor cells, and antibodies to EpCAM are widely used for the isolation and identification of these cancer derived exosomes.
Apart from EpCAM's role in cancer syndromes, homozygous mutations in the EpCAM gene have been identified in patients suffering from congenital tufting enteropathy, a rare autosomal recessive form of intractable diarrhea of infancy.
In addition, EpCAM expression may be causally related to maintaining stem cells, and therefore will be a valuable target for future avenues to generate human induced pluripotent stem cells
In rats, EpCAM (also known as the Gz-1 antigen) is differentially expressed during embryogenesis, from the fertilized rat oocyte to all subsequently arising epithelial structures, suggesting a role for EpCAM in the proliferation and differentiation of embryonic stems cells.
In humans, EpCAM is overexpressed in a wide range of epithelial carcinomas including lung, colorectal, breast, prostate, head and neck, and hepatic carcinomas. The EpCAM molecule has, therefore, been widely used as a marker for human carcinomas and has been considered as a potential target for immunotherapy. EpCAM is also present on exosomes, microvesicles that are abundantly secreted by tumor cells, and antibodies to EpCAM are widely used for the isolation and identification of these cancer derived exosomes.
Apart from EpCAM's role in cancer syndromes, homozygous mutations in the EpCAM gene have been identified in patients suffering from congenital tufting enteropathy, a rare autosomal recessive form of intractable diarrhea of infancy.
In addition, EpCAM expression may be causally related to maintaining stem cells, and therefore will be a valuable target for future avenues to generate human induced pluripotent stem cells
In rats, EpCAM (also known as the Gz-1 antigen) is differentially expressed during embryogenesis, from the fertilized rat oocyte to all subsequently arising epithelial structures, suggesting a role for EpCAM in the proliferation and differentiation of embryonic stems cells.
Product Categories/Family for anti-EpCAM/CD326/EPG-2/SCLC-CD2 antibody
References
1. Schiechl H, Dohr G.1987. Immunohistochemical studies of the distribution of a basolateral-membrane protein in intestinal epithelial cells (GZ1-Ag) in rats using monoclonal antibodies. Histochemistry. 87:491-8.
2. Tarmann T, Dohr G, Schiechl H, Barth S, Hartmann M. 1990. Immunohistochemical detection of an epithelial membrane protein in rat embryos at different stages of development. Acta Anat. 137:141-5.
2. Tarmann T, Dohr G, Schiechl H, Barth S, Hartmann M. 1990. Immunohistochemical detection of an epithelial membrane protein in rat embryos at different stages of development. Acta Anat. 137:141-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,207 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
Epcam
NCBI Official Synonym Symbols
Egp314; Tacstd1
NCBI Protein Information
epithelial cell adhesion molecule; ep-CAM; epithelial glycoprotein 314; tumor-associated calcium signal transducer 1
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
Epcam
UniProt Synonym Gene Names
Tacstd1; Ep-CAM; EGP314
UniProt Entry Name
EPCAM_RAT
NCBI Description
involved in metastasis formation and tumor progression [RGD, Feb 2006]
Uniprot Description
EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.
Protein type: Membrane protein, integral; Cell adhesion; Cell surface; Cell cycle regulation; Motility/polarity/chemotaxis
Cellular Component: tight junction; cell surface; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; lateral plasma membrane
Molecular Function: protein complex binding
Biological Process: ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation; negative regulation of apoptosis
Research Articles on EpCAM/CD326/EPG-2/SCLC-CD2
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Product Notes
The EpCAM/CD326/EPG-2/SCLC-CD2 epcam (Catalog #AAA570318) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti EpCAM/CD326/EPG-2/SCLC-CD2 epithelial antigen reacts with Rat. Does not react with human and may cross-react with other species as described in the data sheet. AAA Biotech's EpCAM/CD326/EPG-2/SCLC-CD2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB). The GZ-20 antibody is suitable for the detection of rat EpCAM by western blotting, immunoprecipitation, immunocytochemistry and flow cytometry. The antibody is also suitable for the detection of rat EpCAM by immunohistochemistry on frozen (acetone fixation for 10 minutes at -20 degree C) and paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration. Researchers should empirically determine the suitability of the EpCAM/CD326/EPG-2/SCLC-CD2 epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EpCAM/CD326/EPG-2/SCLC-CD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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