anti-Human Transferrin Receptor (CD71) Monoclonal Antibody
Mouse anti Transferrin Receptor (CD71)
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Transferrin Receptor (CD71); Monoclonal Antibody; Mouse anti Transferrin Receptor (CD71); anti-Transferrin Receptor (CD71) antibody
Host
Mouse (Balb/c)
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
66IG10
Specificity
66IG10, raised anti Human thymocytes (T-cells) was found to be directed anti the Human transferrin receptor. This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-Transferrin Receptor (CD71) antibody
Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
66IG10 is useful for western blot (on non reduced antigens) and immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titRation.
Source Note
66IG10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with Human T-cells.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-Transferrin Receptor (CD71) antibody
Transferrin receptor protein 1 (TfR1) also known as CD71 (Cluster of Differentiation 71) is a protein that in Humans is encoded by the TFRC gene. CD71 plays a critical role in cell prolifeRation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein. The expression of CD71 is regulated at the post-transcriptional level through the control of mRNA stability and is closely linked to intracellular iron levels. Nitric oxide can affect CD71 expression independently of intracellular iron concentRation by activating proteins which then stabilize CD71 mRNA. Expression of CD71, or the transferrin receptor, is very low on resting leukocytes but is upregulated upon activation reflecting the iron dependence of activation. In other tissues, CD71 is expressed on most dividing cells and also on brain endothelium and alveolar macrophage. The ligand is the Serum iron transport protein transferrin. CD71 antibodies are used as an indiCator of prolifeRation in the typing of lymphomas and leukemias.
Product Categories/Family for anti-Transferrin Receptor (CD71) antibody
References
K. Moolenaar et al. Cancer research 50,1102-1106, Feb 15 1990. Van de Rijna M, Geurts van Kessel AHM, Kroezen V, van Agthoven AJ, Verstijnen K, Terhorst C, Hilgers J: Cytogenet Cell Genet 1983;36:525-531 (DOI: 10.1159/000131967).
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Product Notes
The Transferrin Receptor (CD71) (Catalog #AAA570252) is an Antibody produced from Mouse (Balb/c) and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Transferrin Receptor (CD71) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transferrin Receptor (CD71) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry (IHC) (frozen), Immunoprecipitation (IP), Western Blot (WB). 66IG10 is useful for western blot (on non reduced antigens) and immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titRation. Researchers should empirically determine the suitability of the Transferrin Receptor (CD71) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transferrin Receptor (CD71), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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