Ubc9 recombinant protein
Ubc9 (human), (recombinant) (untagged)
Gene Names
UBE2I; P18; UBC9; C358B7.1
Purity
>=95% (SDS-PAGE)Purified by ion-exchange chromatography
Synonyms
Ubc9; Ubc9 (human); (recombinant) (untagged); Ubc9 recombinant protein
Host
E Coli
Purity/Purification
>=95% (SDS-PAGE)
Purified by ion-exchange chromatography
Purified by ion-exchange chromatography
Form/Format
Liquid. In 20mM HEPES, pH7.3, containing 110mM potassium acetate, 2mM magnesium acetate, 0.8mM EGTA, and 1mM DTT
Source Note
Produced in E Coli as an untagged protein
Preparation and Storage
For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice
Shipping: Shipped on Dry Ice
Related Product Information for Ubc9 recombinant protein
The mechanism for SUMO conjugation is analogous to that of the ubiquitin system. Unlike ubiquitinylation, which leads inter alia to a degradative pathway, SUMO modification of target proteins is involved in nuclear protein targeting, formation of sub-nuclear complexes, regulation of transcriptional activities, and control of protein stability. A short sequence containing the consensus ?-K-X-D/E (where lysine is the modified amino acid, ? is a large hydrophobic residue and X is any amino acid residue) is thought to be necessary for protein SUMOylation to occur however SUMOylation has also been observed in cases where the consensus site is not conserved. Ubc9, the only SUMO E2 enzyme, conjugates activated SUMO (but not ubiquitin) and mediates its subsequent linkage, via C-terminal isopeptide bond formation, to a number of proteins, including RanGAP1, SP100, p53, IkappaBalpha and PML, without the absolute requirement for an E3 ubiquitin-protein ligase-like activity, at least in vitro . Direct interaction of UbcH9 with protein substrates, an unusual feature for an E2 conjugating enzyme, is thought to play a role in substrate recognition.The SUMO E1 activating enzyme heterodimer (Aos1/Uba2) is also required for initiation of the SUMOylation cascade.
Product Categories/Family for Ubc9 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
158
NCBI Official Full Name
SUMO-conjugating enzyme UBC9
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2I
NCBI Official Symbol
UBE2I
NCBI Official Synonym Symbols
P18; UBC9; C358B7.1
NCBI Protein Information
SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Gene Name
UBE2I
UniProt Synonym Gene Names
UBC9; UBCE9
UniProt Entry Name
UBC9_HUMAN
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Product Notes
The Ubc9 ube2i (Catalog #AAA566731) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ubc9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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