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Western Blot (WB)
(Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: HUVEC)
Rabbit UBC9 Monoclonal Antibody | anti-UBC9 antibody
UBC9 Antibody
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
UBC9; Monoclonal Antibody; UBC9 Antibody; C358B7.1 antibody; p18 antibody; SUMO 1 protein ligase antibody; SUMO conjugating enzyme UBC9 antibody; SUMO-conjugating enzyme UBC9 antibody; SUMO-protein ligase antibody; SUMO1 protein ligase antibody; UBC9 antibody; UBC9_HUMAN antibody; UBCE9 antibody; Ube2i antibody; Ubiquitin carrier protein 9 antibody; Ubiquitin carrier protein antibody; Ubiquitin carrier protein I antibody; Ubiquitin conjugating enzyme 9 antibody; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody; Ubiquitin conjugating enzyme E2I (UBC9 homolog; yeast) antibody; Ubiquitin conjugating enzyme UbcE2A antibody; Ubiquitin like protein SUMO 1 conjugating enzyme antibody; Ubiquitin protein ligase E2I antibody; Ubiquitin-conjugating enzyme E2 I antibody; Ubiquitin-protein ligase I antibody; anti-UBC9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC0534
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-UBC9 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: HUVEC)
Western Blot (WB)
(Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: HUVEC)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-UBC9 antibody
UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.
Product Categories/Family for anti-UBC9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
158
NCBI Official Full Name
SUMO-conjugating enzyme UBC9
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2I
NCBI Official Symbol
UBE2I
NCBI Official Synonym Symbols
P18; UBC9; C358B7.1
NCBI Protein Information
SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Gene Name
UBE2I
UniProt Synonym Gene Names
UBC9; UBCE9
UniProt Entry Name
UBC9_HUMAN
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Product Notes
The UBC9 ube2i (Catalog #AAA9435733) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBC9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBC9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the UBC9 ube2i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBC9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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