HDAC1 active protein

HDAC1 (human), (recombinant) (His-tag)

Cat. Num. AAA566657

• Purity: Partially purified by single-step affinity chromatography and gel filtration
• Synonyms: HDAC1; HDAC1 (human); (recombinant) (His-tag); HDAC1 active protein

• Host: Insect cells
• Purity/Purification: Partially purified by single-step affinity chromatography and gel filtration
• Form/Format: Liquid. In 50mM TRIS, pH8.0, 138mM sodium chloride and 10% glycerol

• Source Note: HDAC1 from human cDNA (482 aa). Produced in a baculovirus expression system
• Specific Activity: >=10U/ug. One U=1 pmol/min at 37 degree C, 100uM, FLUOR DE LYS-SIRT1 deacetylase substrate
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for HDAC1 active protein

Human HDAC1 (HD1) was the first protein to be linked to histone deacetylase activity. It is homologous to the yeast protein Rpd31, a relationship which has since come to define the "class I HDACs". HDAC1 promotes transcriptional repression by deacetylating lysine epsilon-amino groups in histone N-terminal tails, a function frequently carried out in association with multi-protein transcription repression complexes such as NuRD3, Sin34 and CoREST6. Ubiquitously expressed in human tissues HDAC1-containing complexes appear to contribute the greater part of (at least class I) deacetylase activity in HeLa nuclear extracts. Aside from its interaction with co-repressors, HDAC1 activity may be regulated by post-translation modifications such as phosphorylation9 and sumoylation or binding to the inhibitor maspin, a tumor-suppressive serpin homolog. Although originally described as a "histone deacetylase", HDAC1 has been shown to catalyze the regulatory deacetylation of non-histone proteins, including p53. Overexpression of HDAC1 has been found in various cancer types. HDAC inhibitors (HDACi) have shown considerable promise as anti-cancer agents and HDACi compounds from multiple chemical classes are in stages of drug development ranging from preclinical to phase III trials.

Product Categories/Family for HDAC1 active protein

Cellular Analysis; Cell Death; Stem Cells; Epigenetics; Histone Posttranslational Modifications; Proteostasis; Posttranslational Modifications; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: Q13547
• Molecular Weight: 55kDa
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1
• UniProt Entry Name: HDAC1_HUMAN

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: Hydrolase; Nuclear receptor co-regulator; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; protein complex; Sin3 complex; cytoplasm; histone deacetylase complex; nuclear chromatin; NuRD complex; chromatin; nucleus; cytosol

Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity

Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis