Mouse VASP Monoclonal Antibody | anti-VASP antibody
VASP (pSer239) monoclonal antibody (16C2) (biotin conjugate)
Does not cross-react with the non-phosphorylated form of VASP
Does not cross-react with the non-phosphorylated form of VASP
ICC: use at 1-10ug/ml; may tolerate 0.5% formaldehyde fixation
IP: use 1-10ug/ per 106 pervanadate-treated A431 cells
WB: 1ug/ml for AP/BCIP/NBT (MTT detection; 0.5ug/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v BSA, 1% (w/v PEG 4,000, 1% (w/v polyvinylpyrrolidone, 0.1% (v/v Tween 20
Reconstituted antibody is stable for 1 year when stored at -80 degree C. Thaw aliquots at +37 degree C. Thawed aliquots may be stored at 4 degree C for up to 3 months
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen
Shipping: Shipped on Blue Ice
NCBI and Uniprot Product Information
NCBI Description
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
Uniprot Description
VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton
Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding
Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization
Research Articles on VASP
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Product Notes
The VASP vasp (Catalog #AAA566322) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VASP (pSer239) monoclonal antibody (16C2) (biotin conjugate) reacts with Human, Mouse, Rat, Rabbit Does not cross-react with the non-phosphorylated form of VASP and may cross-react with other species as described in the data sheet. AAA Biotech's VASP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB). ELISA: use at 0.05ug/ml ICC: use at 1-10ug/ml; may tolerate 0.5% formaldehyde fixation IP: use 1-10ug/ per 106 pervanadate-treated A431 cells WB: 1ug/ml for AP/BCIP/NBT (MTT detection; 0.5ug/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v BSA, 1% (w/v PEG 4,000, 1% (w/v polyvinylpyrrolidone, 0.1% (v/v Tween 20. Researchers should empirically determine the suitability of the VASP vasp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VASP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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