Rabbit anti-Human, Rat Heme oxygenase 2 Polyclonal Antibody | anti-HMOX2 antibody

Heme oxygenase 2 polyclonal antibody

Cat. Num. AAA565778

• Gene Names: HMOX2; HO-2
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G-affinity purified.
• Synonyms: Heme oxygenase 2; Polyclonal Antibody; Heme oxygenase 2 polyclonal antibody; HMOX2; Hsp32; Heat shock protein 32; HO-2; pAb; anti-HMOX2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human and rat heme oxygenase 2.
• Purity/Purification: Protein G-affinity purified.
• Sequence Length: 316

• Applicable Applications for anti-HMOX2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to aa 246-264 of human heme oxygenase 2 (HO-2) protein (E.C. 1.14.99.3).
• Note: The antiserum was raised to a synthetic peptide, sequence: H-E-T-L-E-D-G-F-P-V-H-D-G-K-G-D-M-R-K-C-NH₂ corresponding to amino acid residues 246-264, in amidated form, of the human HO-2 protein (McCoubrey et al., 1992). Two amino acid substitutions (Phe252 --> Leu; Met261 --> Val) exist in the aligned region within rat HO-2, and four (Leu248 --> Val; Gly251 --> Arg; Phe252 --> Leu; Met261 --> Val) in rabbit HO-2. The peptide was conjugated at the C-terminal cysteine residue via a maleimido linkage to keyhole limpet haemocyanin. The antiserum has been purified by caprylic acid and ammonium sulphate precipitation, and by protein G affinity chromatography.; ; Application: Heme oxygenase-2 is constitutively expressed in many tissues, but the protein levels are extremely variable. The use of enriched enzyme preparations is recommended for experimental analysis. This antibody has been shown to react strongly with HO-2 on Western blots of microsomal preparations of unstimulated rat brain (HP 9311), testis (HP 9312) and to a lesser extent with liver (HP 9314). Strong bands have also been detected in extracts of whole rat brain and HepG2, a human hepatocellular carcinoma cell line (# HB8065). A single band at ~36kDa is observed at a primary antibody dilution of 1:2000 or above when detected using enhanced chemiluminescence. Antibody HC 3002 is believed to be wholly specific for HO-2 showing no cross-reactivity with HO-1 in all systems studied to date. The immunostaining is fully abolished by pre-adsorption of the antibody with cognate peptide (HP 9302). The antibody has been used successfully in immunocytochemical studies of HO-2 localisation in human bronchial epithelium biopsy material.; ; Fixatives: Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis: HO-1 was detected using HO-1, pAb (lanes 1, 3, 5 and 7), and HO-2 was detected using HO-2, pAb (lanes 2, 4, 6 and 8). Both antibodies were used at a dilution of 1:1000. Luminograph (ECL, 15 sec. exposure, ref. FM020/054) showing HO-1 and HO-2 expression in lysates prepared from gIFN/LPS stimulated RAW264.7 mouse macrophage cells (lanes 1 and 2), HepG2 (lanes 3 and 4), rat testis (HP9312, lanes 5 and 6) and rat spleen (HP9313, lanes 7 and 8).)

Related Product Information for anti-HMOX2 antibody

Fixatives: Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily.

Product Categories/Family for anti-HMOX2 antibody

Protein Synthesis & Folding; Oxidative Stress

NCBI and Uniprot Product Information

• NCBI GI #: 1170328
• NCBI GeneID: 3163
• UniProt Accession #: P30519; O60605; A8MT35; D3DUD5; I3L430
• Molecular Weight: 32,837 Da
• NCBI Official Full Name: Heme oxygenase 2
• NCBI Official Synonym Full Names: heme oxygenase 2
• NCBI Official Symbol: HMOX2
• NCBI Official Synonym Symbols: HO-2
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Heme oxygenase 2
• Protein Family: Heme oxygenase
• UniProt Gene Name: HMOX2
• UniProt Synonym Gene Names: HO2; HO-2

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane

Molecular Function: heme binding; heme oxygenase (decyclizing) activity; protein binding

Biological Process: cellular iron ion homeostasis; heme catabolic process; heme oxidation; iron ion homeostasis; response to hypoxia; response to oxidative stress