Testing Data
Plasma Fibronectin Native Protein
Human Plasma Fibronectin
Reactivity
Human
Applications
Cell Assay
Synonyms
Plasma Fibronectin; Human Plasma Fibronectin; Plasma Fibronectin native protein
Reactivity
Human
Form/Format
Liquid. 0.15M NaCl, 50mM Tris, pH7.5
Concentration
1mg/ml (varies by lot)
Applicable Applications for Plasma Fibronectin native protein
Cell Assay
Localization
Plasma, 0.3mg/ml extracellular matrix
Mode of action
mediates cell attachment to extracellular matrix
Extinction coefficient
E 1% 1 c m, 280nm= 14.0
Isoelectric point
~5.0
Structure
Heterodimer
Percent carbohydrate
4-10%, depending on source
Preparation and Storage
It is recommended to store the product as single use aliquots at -80 degree C. Thawing should be done slowly at 37 degree C with no agitation. Avoid repeated freeze/thaw cycles.
Shipping: Liquid Nitrogen (Dry Ice)
Shipping: Liquid Nitrogen (Dry Ice)
Testing Data
Testing Data
Related Product Information for Plasma Fibronectin native protein
Fibronectin (cold-insoluble globulin) is a high molecular weight, adhesive, glycoprotein found in both plasma and the extracellular matrix. Fibronectin is composed of two peptide chains of approximately 275,000 molecular weight which are linked through two interchain disulfide bonds at the COOH-terminal end of the molecule. The structure of fibronectin is characterized by three different types of repeating homologous sequence units. The 45 amino acid type-I repeat constitutes the NH2- and COOH-terminal ends of the protein. The two 60 amino acid type-II segments follow the first nine type-I repeats at the NH2-terminus. The 90 amino acid type-III segments occupy the central region of the fibronectin molecule. Structural differences between plasma and cellular fibronectin as well as between the two subunits of plasma fibronectin have been identified. These differences likely originate due to transcriptional and posttranscriptional events involving mRNA splicing.
The apparent function of fibronectin is to mediate cell attachment by interacting with cell surface receptors andextracellular matrix components. Fibronectin contains an Arg-Gly-Asp-Ser (RGDS) cell attachment-promotingsequence within one of the type-III homology segments in the middle of the molecule. This RGDS site is recognizedby specific RGDS-dependent cell receptors which are members of the integrin family of proteins. Fibronectin bindingto activated platelets is mediated through the RGDS cell adhesion sequence. Other binding domains specific for suchextracellular macromolecules as heparin, fibrin(ogen), and collagen have been identified and may be important in thefibronectin-mediated adhesion of platelets to the extracellular matrix of endothelial cells. Fibrin-fibronectin complexesare stabilized by the factor XIIIa-catalyzed covalent cross-linking of fibronectin to a chain of fibrin.
The apparent function of fibronectin is to mediate cell attachment by interacting with cell surface receptors andextracellular matrix components. Fibronectin contains an Arg-Gly-Asp-Ser (RGDS) cell attachment-promotingsequence within one of the type-III homology segments in the middle of the molecule. This RGDS site is recognizedby specific RGDS-dependent cell receptors which are members of the integrin family of proteins. Fibronectin bindingto activated platelets is mediated through the RGDS cell adhesion sequence. Other binding domains specific for suchextracellular macromolecules as heparin, fibrin(ogen), and collagen have been identified and may be important in thefibronectin-mediated adhesion of platelets to the extracellular matrix of endothelial cells. Fibrin-fibronectin complexesare stabilized by the factor XIIIa-catalyzed covalent cross-linking of fibronectin to a chain of fibrin.
Product Categories/Family for Plasma Fibronectin native protein
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Product Notes
The Plasma Fibronectin (Catalog #AAA556506) is a Native Protein and is intended for research purposes only. The product is available for immediate purchase. The Human Plasma Fibronectin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Plasma Fibronectin can be used in a range of immunoassay formats including, but not limited to, Cell Assay. Researchers should empirically determine the suitability of the Plasma Fibronectin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Plasma Fibronectin, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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