Rabbit Synaptophysin (SYP) Polyclonal Antibody | anti-SYP antibody

Synaptophysin (SYP)

Cat. Num. AAA556400

• Gene Names: SYP; MRXSYP
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: Synaptophysin (SYP); Polyclonal Antibody; anti-SYP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Form/Format: Affinity Purified antibody; Lyophilized powder

• Applicable Applications for anti-SYP antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 5-10ug/ml; IF: 5-15ug/ml; WB: 1ug/ml
• Immunogen: Synthetic peptide
• Preparation and Storage: The product can be stored as suppliedfor up to 12 months at 2-4 degree C. After reconstitution, aliquot and store at -20 degree C for higher stability or at 4 degree C with an appropriate antibacterial agent. Avoid freeze thaw-cycles.

Immunohistochemistry (IHC)

(Immunohistochemical detection of Synaptophysin in rat cerebellum. Tissue was fixed with 4% formaldehyde and cut into 10 um thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Synaptophysin at 5ug/mL overnight at 4 degree C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Nuclei of keratinocytes were counterstained with DAPI (blue))

Western Blot (WB)

(Western blot analysis using RA21027)

Related Product Information for anti-SYP antibody

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.

NCBI and Uniprot Product Information

• NCBI GI #: 27764867
• NCBI GeneID: 6855
• NCBI Accession #: NP_003170.1
• NCBI GenBank Nucleotide #: NM_003179.2
• UniProt Accession #: P08247; Q6P2F7; B2R7L6
• Molecular Weight: 33,845 Da
• NCBI Official Full Name: synaptophysin
• NCBI Official Synonym Full Names: synaptophysin
• NCBI Official Symbol: SYP
• NCBI Official Synonym Symbols: MRXSYP
• NCBI Protein Information: synaptophysin; major synaptic vesicle protein P38
• UniProt Protein Name: Synaptophysin
• Protein Family: Synaptophysin
• UniProt Gene Name: SYP
• UniProt Entry Name: SYPH_HUMAN

NCBI Description

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: presynaptic membrane; synaptic vesicle; neuron projection; protein complex; synaptic vesicle membrane; presynaptic active zone; excitatory synapse; cell junction; integral to synaptic vesicle membrane

Molecular Function: identical protein binding; protein self-association; transporter activity; protein complex binding; cholesterol binding; syntaxin-1 binding; SH2 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96