BAI1-Associated 2 Recombinant Protein | BAI1 recombinant protein

Recombinant Human BAI1-Associated 2 Protein

Cat. Num. AAA556298

• Gene Names: BAIAP2; BAP2; FLAF3; IRSP53
• Purity: Greater than 90.0% as determined by: Analysis by SDS-PAGE.
• Synonyms: BAI1-Associated 2; Recombinant Human BAI1-Associated 2 Protein; BAI1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by: Analysis by SDS-PAGE.
• Form/Format: Liquid; The BAIAP2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 30% glycerol.
• Sequence: MSLSRSEEMH RLTENVYKTI MEQFNPSLRN FIAMGKNYEK ALAGVTYAAK GYFDALVKMG ELASESQGSK ELGDVLFQMA EVHRQIQNQL EEMLKSFHNE LLTQLEQKVE LDSRYLSAAL KKYQTEQRSK GDALDKCQAE LKKLRKKSQG SKNPQKYSDK ELQYIDAISN KQGELENYVS DGYKTALTEE RRRFCFLVEK QCAVAKNSAA YHSKGKELLA QKLPLWQQAC ADPSKIPERA VQLMQQVASN GATLPSALSA SKSNLVISDP IPGAKPLPVP PELAPFVGRM SAQESTPIMN GVTGPDGEDY SPWADRKAAQ PKSLSPPQSQ SKLSDSYSNT LPVRKSVTPK NSYATTAENK TLPRSSSMAA GLERNGRMRV KAIFSHAAGD NSTLLSFKEG DLITLLVPEA RDGWHYGESE KTKMRGWFPF SYTRVLDSDG SDRLHMSLQQ GKSSSTGNLL DKDDLAIPPP DYGAASRAFP AQTASGFKQR PYSVAVPAFS QGLDDYGARS MSSGSGTLVS TVVEHHHHHH

• Species: Human
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.15% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for BAI1 recombinant protein

BAIAP2 is a ubiquitous regulator of the actin cytoskeleton. Controled by the Rho-family GTPases BAIAP2 facilitates filopodia development. BAIAP2 is expressed in the cytoplasm and binds small membrane-bound G-proteins to cytoplasmic effector proteins. BAIAP2 was identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is related to an autosomal dominant neurodegenerative disease.

BAIAP2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 530 amino acids (1-522) and having a molecular mass of 58.4kDa.

BAIAP2 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

NCBI and Uniprot Product Information

• NCBI GI #: 73917636
• NCBI GeneID: 10458
• UniProt Accession #: Q9UQB8; O43858; Q53HB1; Q86WC1; Q8N5C0; Q96CR7; Q9UBR3; Q9UQ43
• Molecular Weight: 521
• NCBI Official Full Name: Brain-specific angiogenesis inhibitor 1-associated protein 2
• NCBI Official Synonym Full Names: BAI1-associated protein 2
• NCBI Official Symbol: BAIAP2
• NCBI Official Synonym Symbols: BAP2; FLAF3; IRSP53
• NCBI Protein Information: brain-specific angiogenesis inhibitor 1-associated protein 2; IRS-58; IRSp53/58; fas ligand-associated factor 3; insulin receptor substrate p53/p58; insulin receptor substrate protein of 53 kDa
• UniProt Protein Name: Brain-specific angiogenesis inhibitor 1-associated protein 2
• Protein Family: Brain-specific angiogenesis inhibitor
• UniProt Gene Name: BAIAP2
• UniProt Synonym Gene Names: BAI-associated protein 2; BAI1-associated protein 2; Protein BAP2; FLAF3; IRS-58; IRSp53/58; IRSp53; Insulin receptor substrate p53
• UniProt Entry Name: BAIP2_HUMAN

NCBI Description

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

IRSp53: Adapter protein that links membrane-bound small G- proteins to cytoplasmic effector proteins. Necessary for CDC42- mediated reorganization of the actin cytoskeleton and for RAC1- mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1, BAI1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1. Interacts (via SH3 domain) with E.coli effector protein EspF(U) (via PXXP motifs). Interacts with E.coli intimin receptor Tir. Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleoplasm; ruffle; neuron projection; cytoplasm; plasma membrane; cytosol; actin cytoskeleton; filopodium

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; cytoskeletal adaptor activity

Biological Process: actin crosslink formation; regulation of cell shape; actin filament bundle formation; plasma membrane organization and biogenesis; response to bacterium; axonogenesis; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; dendrite development; regulation of synaptic plasticity; innate immune response; vascular endothelial growth factor receptor signaling pathway