Synaptosomal Associated Protein 25/SNAP25 Blocking Peptide | SNAP25 blocking peptide
Synaptosomal Associated Protein 25/SNAP25
WB: 0.01-0.03 ug/ml
ELISA: 1:32000
Immunohistochemistry Protocol
Tissue Preparation: Formalin fixation and embedding in paraffin wax. Tissue Sectioning: Make 4-mum sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60 degree C.
Deparaffinization: Wash dry slides in 3 changes of xylene-5 minutes each at Room Temperature.
Rehydration: Wash slides in 3 changes of 100% alcohol-3 minutes each at Room Temperature. Wash slides in 2 changes of 95% alcohol-3 minutes each at Room Temperature Wash slides in 1 change of 80% alcohol-3 minutes at Room Temperature. Rinse slides in gentle running distilled water-5 minutes at Room Temperature.
Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100 degree C-20 minutes. Remove from heat and let stand at room temperature in buffer-20 minutes. Rinse in 1X TBS with Tween (TBST)-1 minute at Room Temperature.
Immunostaining: (Do not allow tissues to dry at any time during the staining procedure). Apply a universal protein block-20 minutes at Room Temperature. Drain protein block from slides, apply diluted primary antibody-45 minutes at Room Temperature. Rinse slides in 1X TBST-1 minute at Room Temperature. Apply a biotinylated anti-rabbit IgG (H+L) secondary-30 minutes at Room Temperature. Rinse slides in 1X TBST-1 minute at Room Temperature. Apply alkaline phosphatase streptavidin-30 minutes at Room Temperature. Rinse slides in 1X TBST-1 minute at Room Temperature. Apply alkaline phosphatase chromogen substrate-30 minutes at Room Temperature. Wash slides in distilled water-1 minute at Room Temperature
Dehydrate: (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB). Wash slides in 2 changes of 80% alcohol-1 minute each at Room Temperature. Wash slides in 2 changes of 95% alcohol-1 minute each at Room Temperature. Wash slides in 3 changes of 100% alcohol-1 minute each at Room Temperature. Wash slides in 3 changes of xylene-1 minute each at Room Temperature. Apply coverslip
Short term: +4 degree C.
Repeated freeze/thaw cycles compromise the integrity of the antibody.
NCBI and Uniprot Product Information
NCBI Description
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein. It is essential for regulated exocytosis in neuronal cells. Alternative transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed:8243676, PubMed:8103915). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (PubMed:12403834). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Research Articles on SNAP25
Similar Products
Product Notes
The SNAP25 snap25 (Catalog #AAA555837) is a Blocking Peptide produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Synaptosomal Associated Protein 25/SNAP25 reacts with Human and may cross-react with other species as described in the data sheet. IHC: 2.5 ug/ml WB: 0.01-0.03 ug/ml ELISA: 1:32000. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptosomal Associated Protein 25/SNAP25, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
