Immunofluorescence (IF)
(Image: Human SH-SY5Y cells stained with Fibrillarin/Nop1p showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Cells are also stained with Neurofilament NF-H.)
Mouse Fibrillarin/Nop1p nucleolar marker Antibody
Fibrillarin/Nop1p nucleolar marker, Monoclonal Antibody, Mouse
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Protein G purified
Synonyms
Fibrillarin/Nop1p nucleolar marker; Monoclonal Antibody; Mouse; anti-Fibrillarin/Nop1p nucleolar marker antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG1
Purity/Purification
Protein G purified
Form/Format
Sterile-filtered cell culture fluid from an Integra CL-350 bio-chamber with10 mM sodium azideas a preservative.
Applicable Applications for anti-Fibrillarin/Nop1p nucleolar marker antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Western Blot: 1:500 (cell lysates)-1,10,000 (nuclear fractions)
For western blots of yeast protein samples, use antibodydiluted 1/2,000 (cell lysates) to 1/10,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) westerndetection methods, trydiluted 1/1,000 to 1/5,000. To detect mammalian fibrillarin on western blots by ECL, tryat 1/500 dilution.
Immunofluorescence: 1:500-1:5,000
For mammalian cells, start dilution at 1:500
Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
For western blots of yeast protein samples, use antibodydiluted 1/2,000 (cell lysates) to 1/10,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) westerndetection methods, trydiluted 1/1,000 to 1/5,000. To detect mammalian fibrillarin on western blots by ECL, tryat 1/500 dilution.
Immunofluorescence: 1:500-1:5,000
For mammalian cells, start dilution at 1:500
Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Immunogen
Mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.
Preparation and Storage
Antibody can be aliquotted and stored frozen at -20°C to -70°C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2°-8°C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Image: Human SH-SY5Y cells stained with Fibrillarin/Nop1p showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Cells are also stained with Neurofilament NF-H.)
Immunofluorescence (IF)
(Image: Human SH-SY5Y cells stained with Fibrillarin/Nop1p showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Cells are also stained with Neurofilament NF-H.)
Western Blot (WB)
(Image: Strip blots of yeast protein extracts stained with the indicated Fibrillarin/Nop1p and related markers.)
Western Blot (WB)
(Image: Strip blots of yeast protein extracts stained with the indicated Fibrillarin/Nop1p and related markers.)
Related Product Information for anti-Fibrillarin/Nop1p nucleolar marker antibody
The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiaeNop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein.This protein lacks the RGG richN-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The 3D structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes (Medline link). Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient markerfor nucleoli in a wide variety of species (e.g. 4-6).
Product Categories/Family for anti-Fibrillarin/Nop1p nucleolar marker antibody
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Product Notes
The Fibrillarin/Nop1p nucleolar marker (Catalog #AAA555050) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fibrillarin/Nop1p nucleolar marker, Monoclonal Antibody, Mouse reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibrillarin/Nop1p nucleolar marker can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western Blot: 1:500 (cell lysates)-1,10,000 (nuclear fractions) For western blots of yeast protein samples, use antibodydiluted 1/2,000 (cell lysates) to 1/10,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) westerndetection methods, trydiluted 1/1,000 to 1/5,000. To detect mammalian fibrillarin on western blots by ECL, tryat 1/500 dilution. Immunofluorescence: 1:500-1:5,000 For mammalian cells, start dilution at 1:500 Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Researchers should empirically determine the suitability of the Fibrillarin/Nop1p nucleolar marker for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibrillarin/Nop1p nucleolar marker, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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