Interleukin-20 Recombinant Protein | IL-20 recombinant protein

Human Interleukin-20 Recombinant

Cat. Num. AAA553328

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-20; Human Interleukin-20 Recombinant; Human IL-20 recombinant; TRF; il20; il-20; interleukin-17; IL-20 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Human Interleukin-17 was lyophilized from a 0.2 um filtered PBS solution pH 7.5.
• Sequence Length: 176

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human Interleukin-20 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin-20 generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa.; ; Recombinant IL-20 is a disulfide-linked monomeric protein consisting of 135 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-20 recombinant protein

L-19 and IL-20 are mainly produced by activated monocytes, IL-24 is produced by Th2 cells, monocytes, and melanocytic cells, and IL-22 and IL-26 are primarily produced by activated T cells. Like IL- 10, these class II cytokines signal through heterodimeric receptors. Overexpression of IL-20 in transgenic mice causes neonatal death as well as skin abnormalities, including aberrant epidermal differentiation. IL-20 selectively enhances multipotential hematopoietic progenitors in vitro and in vivo. IL-20 is preferentially expressed in monocytes18 and induces STAT 3 activation through binding to 2 types of IL-20 receptor (R) complexes, either IL- 20R1/IL-20R2 or IL-22R1/IL- 20R2

Product Categories/Family for IL-20 recombinant protein

Cytokines

References

• anti–IL-20 monoclonal antibody alleviates inflammation in oral cancer and suppresses tumor growth .Mol. Cancer Res., Nov 2012; 10: 1430 - 1439.
• anti–IL-20 monoclonal antibody suppresses breast cancer progression and bone osteolysis in murine models .J. Immunol., Feb 2012; 188: 1981 - 1991.
• expression of IL-20 in lesional skin and synovium of patients with psoriatic arthritis compared to rheumatoid arthritis and its response to alefacept treatment .Ann Rheum Dis, Feb 2012; 71: A15.
• anti–IL-20 monoclonal antibody inhibits the differentiation of osteoclasts and protects against osteoporotic bone loss .J. Exp. Med., Aug 2011; 208: 1849 - 1861.
• IL-20 blockade prevents the upregulation of ccl2, ccl4, ccl5 and ccr7 by ra synovial biopsies .Ann Rheum Dis, Mar 2011; 70: A76 - A77.