MEC (CCL28) Recombinant Protein | CCL28 recombinant protein

Mouse MEC (CCL28) Recombinant

Cat. Num. AAA553284

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: MEC (CCL28); Mouse MEC (CCL28) Recombinant; Human MECK Recombinant; mec; ccl28; SCYA28; CCL28 recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CCL-28 was lyophilized from PBS pH 7.4.
• Sequence: AAVILHVKRR RICISPHNRT LKQWMRASEV KKNGRENVCS GKKQPSRKDR KGHTTRKHRT RGTHRHEASR
• Sequence Length: 130

• Domain: IL8
• Host Note: Optimized DNA sequence encoding mouse CCL28 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native mouse MEC/CCL28 has a calculated molecular mass of approximately 12 kDa. ; ; Recombinant CCL28 is a disulfide-linked homodimeric protein consisting of 111 amino acid residue subunits, and migrates as an approximately 12 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CCL28 recombinant protein

The mucosa-associated epithelial chemokine CCL28 (mucosa-associated epithelial chemokine) is a common mucosal chemokine which is constitutively expressed by epithelial cells in most mucosal sites. Both CCL28 and CCL25 have recently been shown to be essential for lymphocyte migration to gastrointestinal tissues. CCL28 attracts IgA ASC, but not IgG or IgM ASC, from both intestinal and extraintestinal mucosal tissue, while CCL25 preferentially attracts IgA ASC from the small intestine and its draining lymphoid tissues, as well as α4β7+ T cells.

Product Categories/Family for CCL28 recombinant protein

Cytokines

References

• mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts ccr10+ iga plasma cells following mucosal vaccination via estrogen control .J. Immunol., Sep 2011; 187: 3044 - 3052.
• CCL28 is increased in human helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin a-secreting cells .Infect. Immun., Jul 2008; 76: 3304 - 3311.
• ccl25 and CCL28 promote 4β7-integrin-dependent adhesion of lymphocytes to madcam-1 under shear flow .Am J Physiol Gastrointest Liver Physiol, May 2008; 294: G1257 - G1267.
• epithelial inflammation is associated with CCL28 production and the recruitment of regulatory t cells expressing ccr10 .J. Immunol., Jul 2006; 177: 593 - 603.
• redundant role of chemokines ccl25/teck and CCL28/mec in iga+ plasmablast recruitment to the intestinal lamina propria after rotavirus infection .J. Immunol., May 2006; 176: 5749 - 5759.