Interleukin-8 (77AA) Recombinant Protein | IL-8 recombinant protein

Human Interleukin-8 (77AA) Recombinant

Cat. Num. AAA553216

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-8 (77AA); Human Interleukin-8 (77AA) Recombinant; il-8; il8; NAP-1; nap1; cxcl8; IL-8 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Interleukin-8 was lyophilized from a 0.2 um filtered PBS solution pH 7.0.
• Sequence: MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
• Sequence Length: 99

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human Interleukin-8, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 9 kDa. Recombinant Interleukin-8 is a disulfide-linked monomer protein consisting of 78 amino acid residue subunits, migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-8 recombinant protein

IL8 is produced by stimulated monocytes but not by tissue macrophages and T-lymphocytes. In many cell types the synthesis of IL8 is strongly stimulated by IL1 and TNF-alpha. In human skin fibroblasts the expression of IL-8 is enhanced by Leukoregulin.The expression of IL-8 from resting and stimulated human blood monocytes is up-regulated by IL-7. In chondrocytes the synthesis of IL8 is stimulated by IL1-beta TNF-alpha and bacterial lipopolysaccharides. In human astrocytes the synthesis and secretion of IL8 is induced by IL-1 and TNF-alpha. The IL8 receptor is a dimeric glycoprotein consisting of a 59 kDa and a 67 kDa subunit. It has been given the name CD128. It is expressed in many different cell types including those not responding to IL8. The biological activities of IL8 resemble those of a related protein, NAP-2. IL8 differs from all other cytokines in its ability to specifically activate neutrophil granulocytes. In neutrophils IL8 causes a transient increase in cytosolic calcium levels and the release of enzymes from granules. IL-8 also enhances the metabolism of reactive oxygen species and increases Chemotaxis and the enhanced expression of adhesion molecules. A pre-activation by IL3 is required to render basophils and neutrophils susceptible to further activation by IL8.

Product Categories/Family for IL-8 recombinant protein

Cytokines

References

• cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/IL-8 cytokine network .PNAS, Sep 2009; 10.1073/pnas.0905299106.
• the angiogenic effect of probiotic bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8 .Am J Physiol Gastrointest Liver Physiol, Sep 2009; 10.1152/ajpgi.00204.2009.
• IL-8 signaling does not mediate intra-amniotic lps-induced inflammation and maturation in preterm fetal lamb lung .Am J Physiol Lung Cell Mol Physiol, Sep 2009; 297: L512 - L519.
• the functional significance behind expressing two IL-8 receptor types on pmn .J. Leukoc. Biol., Sep 2009; 86: 529 - 543.
• IL-8 regulates mucin gene expression at the posttranscriptional level in lung epithelial cells .J. Immunol., Aug 2009; 183: 2159 - 2166.