Keratinocyte Growth Factor Recombinant Protein | KGF recombinant protein

Human Keratinocyte Growth Factor Recombinant

Cat. Num. AAA553202

• Purity: >96%, as determined by SDS-PAGE and HPLC
• Synonyms: Keratinocyte Growth Factor; Human Keratinocyte Growth Factor Recombinant; KGF; FGF7; FGF-7; KGF recombinant protein

• Host: E Coli
• Purity/Purification: >96%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant KGF was lyophilized from a 0.2 um filtered 1M NaCl, 20mM PB solution pH 8.0.
• Sequence: MHKWILTWIL PTLLYRSCFH IICLVGTISL ACNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT
• Sequence Length: 194

• Domain: IL1
• Host Note: Optimized DNA sequence encoding Human Keratinocyte Growth Factor mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human KGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant Keratinocyte Growth Factor is a disulfide-linked monomeric protein consisting of 164 amino acid residue subunits. Interefron alpha 2b migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for KGF recombinant protein

KGF is a member of the family of fibroblast growth factors, it is secreted in large amounts by fibroblast- like stromal cells in epithelial tissues. The KGF receptor encodes a tyrosine kinase and is a member of the family of receptors binding aFGF and bFGF. Binding of KGF to its receptor is competed by aFGF and bFGF. Although KGF is a potent specific mitogen for many epithelial cells it is not so for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF.

Product Categories/Family for KGF recombinant protein

Cytokines

References

• influence of KGF on the progression of pancreatic cancer .Anticancer Res, Aug 2009; 29: 3417 - 3420.
• keratinocyte growth factor (KGF) regulates estrogen receptor- (er-) expression and cell apoptosis via phosphatidylinositol 3-kinase (pi3k) /akt pathway in human breast cancer cells .Anticancer Res, Aug 2009; 29: 3195 - 3205.
• KGF and egf signalling block hair follicle induction and promote interfollicular epidermal fate in developing mouse skin .Development, Jul 2009; 136: 2153 - 2164.
• sirna inhibition of er- expression reduces KGF-induced proliferation of breast cancer cells .Anticancer Res, Sep 2008; 28: 2733 - 2735.
• effects of KGF on alveolar epithelial cell transdifferentiation are mediated by jnk signaling .Am. J. Respir. Cell Mol. Biol., Feb 2008; 38: 239 - 246.