Interferon-beta 1a Recombinant Protein | IFN-beta recombinant protein

Human Interferon-beta 1a Recombinant

Cat. Num. AAA553062

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: Interferon-beta 1a; Human Interferon-beta 1a Recombinant; ifn; ifnb; interferon beta; ifn-beta; IFN-beta recombinant protein

• Host: Chinese Hamster Ovary Cells (CHO)
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant IFN-b 1a was lyophilized from a 0.2 um filtered sodium acetate solution pH 4.8.
• Sequence: MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN
• Sequence Length: 187

• Domain: Four Helix Cytokine Core
• Host Note: Optimized DNA sequence encoding Human Interferon beta 1a mature chain was expressed in Chinese Hamster Ovary Cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Recombinant human IFN-beta 1a, generated by the proteolytic removal of the signal peptide and propeptide, and has a calculated molecular mass of approximately 19 kDa. ; ; Recombinant Interferon beta is a disulfide-linked monomeric protein consisting of 166 amino acid residue subunits, and migrates due to glycosylation as an approximately 22 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IFN-beta recombinant protein

IFN-beta is produced mainly by fibroblasts and some epithelial cell types. The synthesis of IFN-beta can be induced by common inducers of interferons including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL1. IFN-beta binds to the same receptor as IFN-alpha. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class 1 antigens and blocks the expression of HLA class 2 antigens stimulated by IFN-gamma. IFN-beta stimulates the activity of NK-cells and hence also antibody-dependent cytotoxicity. IFN-beta shows antiproliferative activity against a number of cell lines established from solid tumors.

Product Categories/Family for IFN-beta recombinant protein

Cytokines

References

• randomized study of interferon beta-1a, low-dose azathioprine, and low-dose corticosteroids in multiple sclerosis .Multiple Sclerosis, Aug 2009; 15: 965 - 976.
• effect of statins on clinical and molecular responses to intramuscular interferon beta-1a .Neurology, Jun 2009; 72: 1989 - 1993.
• efficacy of intramuscular interferon beta-1a in patients with clinically isolated syndrome: analysis of subgroups based on new risk criteria .Multiple Sclerosis, Jun 2009; 15: 728 - 734.
• subcutaneous interferon beta-1a has a positive effect on cognitive performance in mildly disabled patients with relapsing—remitting multiple sclerosis: 2-year results from the cogimus study .Therapeutic Advances in Neurological Disorders, Mar 2009; 2: 67 - 77.
• safety and immunogenicity of a new formulation of interferon β-1a (rebif® new formulation) in a phase iiib study in patients with relapsing multiple sclerosis: 96-week results .Multiple Sclerosis, Feb 2009; 15: 219 - 228.