CD200 (MOX1) Recombinant Protein | CD200 recombinant protein
Human CD200 Recombinant (MOX1)
Purity
>98%, as determined by SDS-PAGE and HPLC
Synonyms
CD200 (MOX1); Human CD200 Recombinant (MOX1); Human CD200 Recombinant (MOX1) ; CD200; MOX1; MOX2; MRC; OX-2; CD200 recombinant protein
Host
HEk293 Cells
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CD200 is supplied as a 0.2 um filtered PBS solution, pH7.2.
Sequence Length
294
Domain
Ig-like C2
Host Note
Optimized DNA sequence encoding Human extracellular domain of human CD200 including a C-terminal 6His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Weight Note
Recombinant CD200 is a monomer protein consisting of 213 amino acid residue subunits, due to glycosylation migrates as an approximately 40-45kDa protein on SDS-PAGE.
Preparation and Storage
Recombinant CD200, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for CD200 recombinant protein
CD200 is a member of the immunoglobulin type 1 superfamily that is highly expressed in the central nervous system, dendritic cells, and lymphocytes. CD200 is functionally involved in an immunosuppressive signaling pathway via interaction with its receptor, CD200R, with downstream effects of macrophage inhibition, induction of regulatory T cells, and inhibition of tumor-specific T cells. In addition to being expressed in normal tissues, CD200 has also been demonstrated in solid tumors5 and hematologic malignancies, such as acute leukemias, chronic lymphocytic leukemia/small lymphocytic lymphoma, hairy cell leukemia, mediastinal large B-cell lymphoma, classic Hodgkin lymphoma, and angioimmunoblastic T-cell lymphoma. In addition, neoplastic plasma cells frequently upregulate CD200 expression, as demonstrated by the large proportion of CD200+ plasma cell myeloma (PCM) cases (approximately 70%) found on gene expression profiling and flow cytometry. In contrast, normal plasma cells are CD200.
Product Categories/Family for CD200 recombinant protein
References
• CD200 is preferentially expressed in cd138 dim multiple myeloma cells: implications for a myeloma stem cell subpopulation with potential immune-resistance. .Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 4877.
• essential roles for dok2 and rasgap in CD200 receptor-mediated regulation of human myeloid cells .J. Immunol., Oct 2009; 183: 4879 - 4886.
• lack of CD200 enhances pathological t cell responses during influenza infection .J. Immunol., Aug 2009; 183: 1990 - 1996.
• expression of a CD200 transgene is necessary for induction but not maintenance of tolerance to cardiac and skin allografts .J. Immunol., Aug 2009; 183: 1560 - 1568.
• induction of CD200 signalling reduces inflammatory cell infiltration and inhibits laser-induced choroidal neovascularization .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 3759.
• essential roles for dok2 and rasgap in CD200 receptor-mediated regulation of human myeloid cells .J. Immunol., Oct 2009; 183: 4879 - 4886.
• lack of CD200 enhances pathological t cell responses during influenza infection .J. Immunol., Aug 2009; 183: 1990 - 1996.
• expression of a CD200 transgene is necessary for induction but not maintenance of tolerance to cardiac and skin allografts .J. Immunol., Aug 2009; 183: 1560 - 1568.
• induction of CD200 signalling reduces inflammatory cell infiltration and inhibits laser-induced choroidal neovascularization .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 3759.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,264 Da
NCBI Official Full Name
OX-2 membrane glycoprotein isoform b
UniProt Protein Name
OX-2 membrane glycoprotein
Protein Family
UniProt Gene Name
CD200
UniProt Synonym Gene Names
MOX1; MOX2
UniProt Entry Name
OX2G_HUMAN
NCBI Description
The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CD200: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: regulation of immune response
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Product Notes
The CD200 cd200 (Catalog #AAA553059) is a Recombinant Protein produced from HEk293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD200 (MOX1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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