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Immunohistochemistry (IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat kidney sections showing lymphocyte expression of CD3. It was used as primary antibody, dilution 1:200, DAB chromogenic staining. )

CD3 Polyclonal Antibody | anti-CD3 antibody

Rabbit anti CD3 Polyclonal

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purity: >95%

Purification: Serum IgG fraction was purified by Protein G affinity chromatography.
Synonyms
CD3; Polyclonal Antibody; Rabbit anti CD3 Polyclonal; Rabbit anti Human CD3 Polyclonal purified; cd3; cd-3; polyclonal; PAb; anti-CD3 antibody
Ordering
For Research Use Only!
Host
Polyclonal anti CD3 was produced from sera of rabbits immunized with synthetic protein containing CD3 antigenic determinats. Recognizes the 20kD epsilon chain of the CD3 complex.
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purity: >95%

Purification: Serum IgG fraction was purified by Protein G affinity chromatography.
Form/Format
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
Sequence Length
207
Applicable Applications for anti-CD3 antibody
Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)
Application Notes
Western Blot: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IF: A suitable range of concentrations of this antibody for IHC is 10-20 ug/ml.
Immunoflourescence analysis of CD3-expressison in Human colon cancer tissue
It was used as primary antibody, dilution 1:60.
Source Note
Polyclonal CD3 antibody was produced from sera of rabbits immunized with synthetic protein containing CD3 antigenic determinants.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Presentation
Lyophilzed from 0.2um filtered solution in PBS pH 7.4.
Dilution
A quick spin of the vial followed by reconstitution 0.01M PBS pH 7.4 or other diluents.
Preparation and Storage
The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months

Immunohistochemistry

(IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat kidney sections showing lymphocyte expression of CD3. It was used as primary antibody, dilution 1:200, DAB chromogenic staining. )

Immunohistochemistry (IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat kidney sections showing lymphocyte expression of CD3. It was used as primary antibody, dilution 1:200, DAB chromogenic staining. )

Test Data

( Immunoflourescence of human colon cancer sections showing expression of CD3. MBS551029 was used as a primary antibody , dilution 1:60.)

Test Data ( Immunoflourescence of human colon cancer sections showing expression of CD3. MBS551029 was used as a primary antibody , dilution 1:60.)
Related Product Information for anti-CD3 antibody
The membrane glycoprotein CD34 has been proven to be a useful marker for human hematopoietic stem/progenitor cells, and various types of colony- forming activity in human bone marrow, cord blood, and mobilized peripheral blood are contained in the CD34+ population.
Product Categories/Family for anti-CD3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
916
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,147 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
Protein Family
UniProt Gene Name
CD3E
UniProt Synonym Gene Names
T3E
UniProt Entry Name
CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; T cell receptor signaling pathway; negative thymic T cell selection; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell anergy; response to nutrient; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 18

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Product Notes

The CD3 cd3e (Catalog #AAA551029) is an Antibody produced from Polyclonal anti CD3 was produced from sera of rabbits immunized with synthetic protein containing CD3 antigenic determinats. Recognizes the 20kD epsilon chain of the CD3 complex. and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD3 Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). Western Blot: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IF: A suitable range of concentrations of this antibody for IHC is 10-20 ug/ml. Immunoflourescence analysis of CD3-expressison in Human colon cancer tissue It was used as primary antibody, dilution 1:60. Researchers should empirically determine the suitability of the CD3 cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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