Sandwich ELISA
(96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.For Sandwich ELISA image: capture antibody ()
Goat anti-Human TPO Polyclonal Antibody | anti-TPO antibody
TPO Antibody
Sandwich ELISA
(96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.For Sandwich ELISA image: capture antibody ()
Sandwich ELISA
(96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.For Sandwich ELISA image: capture antibody ()
Western Blot (WB)
(The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20, the membrane was incubated with primary antibody (at 4°C overnight.Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.Enhanced chemiluminescence (ECL) reagents were used to visualize.Recombinant immunogen ()
Western Blot (WB)
(The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20, the membrane was incubated with primary antibody (at 4°C overnight.Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.Enhanced chemiluminescence (ECL) reagents were used to visualize.Recombinant immunogen ()
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
Research Articles on TPO
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Product Notes
The TPO (Catalog #AAA550367) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The TPO Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPO can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the TPO for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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