Interleukin-31 Active Protein | IL31 active protein

RecombinantHuman Interleukin-31 (rHu IL-31)

Cat. Num. AAA546163

• Gene Names: IL31; IL-31
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Synonyms: Interleukin-31; RecombinantHuman Interleukin-31 (rHu IL-31); human IL-31; Human Interleukin-31; IL31 active protein

• Host: E Coli
• Purity/Purification: >97% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2mm filtered concentrated solution in PBS.; Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 164

• Molecular Weight Information: Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids
• Biological Activity: Fully biologically active when compared to standard.Measured by its ability to induce STAT3 activation in U-87 MG human glioblastoma/astrocytoma cells. 5 ng/mL of rhIL31 can effectively induce STAT3 activation.
• Endotoxin Level: Less than 1EU/mg of rHuIL-31 as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for IL31 active protein

Human IL-31 is a T-cell derived cytokine that shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. It signals through a receptor complex comprised of GPL (GP130-like, IL- 31RA) and OSMR (Oncostatin M receptor). GPL/OSMR signaling is a strong activator of STAT3 and STAT5, and can also activate STAT1, Jak1, and Jak2 signaling pathways. IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 62122911
• NCBI GeneID: 386653
• NCBI Accession #: NP_001014358.1
• NCBI GenBank Nucleotide #: NM_001014336.1
• UniProt Accession #: Q6EBC2; A2RUQ1
• Molecular Weight: 18,205 Da
• NCBI Official Full Name: interleukin-31
• NCBI Official Synonym Full Names: interleukin 31
• NCBI Official Symbol: IL31
• NCBI Official Synonym Symbols: IL-31
• NCBI Protein Information: interleukin-31
• UniProt Protein Name: Interleukin-31
• Protein Family: Interleukin
• UniProt Gene Name: IL31
• UniProt Synonym Gene Names: IL-31
• UniProt Entry Name: IL31_HUMAN

NCBI Description

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]

Uniprot Description

IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: immune system process