Klotho Active Protein | KL active protein

Klotho protein

Cat. Num. AAA539406

• Applications: User optimized
• Purity: > 98% pure
• Synonyms: Klotho; Klotho protein; aKlotho protein; KLA protein; KL active protein

• Host: CHO cells
• Purity/Purification: > 98% pure
• Form/Format: Lyophilized from 15mM Sodium Phosphate pH 7.3 0.2 micron filtered. Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.
• Concentration: 20 ug/vial; lyophilized (varies by lot)
• Sequence: EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH.

• Applicable Applications for KL active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid.
• Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Mouse NIH-3T3 cells. Recombinant human Klotho is effective in a concentration range of 0.5-2.0 ug/ml.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Species: Human
• Additional Remarks: Effective at a concentration of 0.5-2.0 ug/ml
• Preparation and Storage: Ships ambient of refrigerated. Upon receipt store at 4 degree C short term, -20 degree C long term. After reconstituted, the solution can be stored at 4 degree C for up to 1 week. Working aliquots stored with a carrier protein are stable for at least 3 months at -20 degree C. Avoid repeated freeze-thaw cycles.

Related Product Information for KL active protein

Purified recombinant Human Klotho protein

Product Categories/Family for KL active protein

Nutrition & Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 2618596
• NCBI GeneID: 9365
• UniProt Accession #: Q9UEF7; Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0
• Molecular Weight: 62,135 Da
• NCBI Official Full Name: klotho
• NCBI Official Synonym Full Names: klotho
• NCBI Official Symbol: KL
• NCBI Protein Information: klotho
• UniProt Protein Name: Klotho
• Protein Family: Klotho
• UniProt Gene Name: KL
• UniProt Entry Name: KLOT_HUMAN

NCBI Description

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

Uniprot Description

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.2.1.31; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular space; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: vitamin D binding; signal transducer activity; beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; hormone activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial