TMB substrate
TMB Membrane Substrate
Applications
ELISA
Purity
Highly pure
Synonyms
TMB; TMB Membrane Substrate; TMB substrate
Purity/Purification
Highly pure
Form/Format
Supplied as a liquid.
Applicable Applications for TMB substrate
ELISA (EIA)
Application Notes
Optimal conditions to be determined by end user. The product should be allowed to equilibrate to room temperature (25 degree C) prior to use. For use, completely cover the membrane surface with substrate. The substrate system will react with sites on the membrane containing peroxidase producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. The reaction should be monitored and read before the background color becomes to intense which may result in diminished contrast between the positive and background staining. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
Biological Significance
This HRP Membrane Substrate is a ready to use solution containing 3,3?,5,5?- tetramethylbenzidine in a mildly acidic buffer. Unreacted substrate should be faint yellow to pink in appearance. When this substrate system is reacted with peroxidase, an insoluble permanent dark blue reaction product is obtained. This substrate is not recommended for microwell or immunohistochemical applications.
Preparation and Storage
Store at 4 degree C.
Related Product Information for TMB substrate
High sensitivity HRP membrane substrate (containing TMB)
Product Categories/Family for TMB substrate
Similar Products
Product Notes
The TMB (Catalog #AAA539296) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TMB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Optimal conditions to be determined by end user. The product should be allowed to equilibrate to room temperature (25 degree C) prior to use. For use, completely cover the membrane surface with substrate. The substrate system will react with sites on the membrane containing peroxidase producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. The reaction should be monitored and read before the background color becomes to intense which may result in diminished contrast between the positive and background staining. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted. Researchers should empirically determine the suitability of the TMB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMB, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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