PDGF AA active protein
PDGF AA protein
Gene Names
pdgfa; pdgf; pdgf-a
Applications
User optimized
Purity
> 98% pure
Synonyms
PDGF AA; PDGF AA protein; Platelet-Derived Growth Factor-AA protein; ODGF protein; GDGF protein; Glioma-derived growth factor protein; Osteosarcoma-derived Growth Factor protein; PDGF AA active protein
Host
Human
Purity/Purification
> 98% pure
Form/Format
Supplied lyophilized with no additives. Reconstitute in 10mM CH3COOH to a concentration of 0.1 - 1.0 mg/ml.
Sequence
Region of PDGF AA protein corresponding to amino acids SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT.
Sequence Length
226
Applicable Applications for PDGF AA active protein
User optimized
Protein Type
Recombinant
Biological Significance
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms.
Bioactivity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is approximately 1 ng/ml, corresponding to a specific activity of approximately 1 x 10^6 units/mg.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for PDGF AA active protein
Purified recombinant Human PDGF AA protein
Product Categories/Family for PDGF AA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28.5 kDa
NCBI Official Full Name
platelet-derived growth factor subunit A
NCBI Official Synonym Full Names
platelet-derived growth factor alpha polypeptide<
NCBI Official Symbol
pdgfa
NCBI Official Synonym Symbols
pdgf; pdgf-a
NCBI Protein Information
platelet-derived growth factor subunit A
UniProt Protein Name
Platelet-derived growth factor subunit A
UniProt Gene Name
pdgfa
UniProt Synonym Gene Names
PDGF subunit A
UniProt Entry Name
PDGFA_XENLA
Uniprot Description
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Signaling is modulated by the formation of heterodimers with PDGFB ().
Research Articles on PDGF AA
Similar Products
Product Notes
The PDGF AA pdgfa (Catalog #AAA537772) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDGF AA can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the PDGF AA pdgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Region of PDGF AA protein correspond ing to amino acids SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT. It is sometimes possible for the material contained within the vial of "PDGF AA, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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