Keratin K8 Native Protein | KRT8 native protein

Keratin K8 protein

Cat. Num. AAA536603

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Applications: User optimized
• Purity: > 95% pure
• Synonyms: Keratin K8; Keratin K8 protein; CYK8 protein; KO protein; Keratin 8 protein; K2C8 protein; K8 protein; CARD2 protein; CK8 protein; Keratin K 8 protein; Keratin K-8; Keratin K-8 protein; Cytokeratin 8 protein; Keratin K 8; KRT8 protein; KRT8 native protein

• Host: Bovine
• Purity/Purification: > 95% pure
• Form/Format: Supplied in lyophilized form with 30 mM Tris/HCI pH 8, 9 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for KRT8 native protein: User optimized
• Source Note: Bovine liver
• Protein Type: Native
• Biological Significance: Keratin, type I cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human is encoded by the KRT8 gene. It is often paired with keratin 18. Antibodies to CK8 (e.g. CAM 5.2) can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
• Preparation and Storage: Store at 2-8 degree C in lyophilized form. Following reconstitution aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for KRT8 native protein

Purified native Bovine Keratin K8 Protein

Product Categories/Family for KRT8 native protein

Cell Biology; Native Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4504919
• NCBI GeneID: 3856
• NCBI Accession #: NP_002264.1
• NCBI GenBank Nucleotide #: NM_002273.3
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
• Molecular Weight: 52 kDa
• NCBI Official Full Name: keratin, type II cytoskeletal 8 isoform 2
• NCBI Official Synonym Full Names: keratin 8, type II
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; nuclear matrix; costamere; cytoplasm; keratin filament; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; response to other organism; sarcomere organization; response to hydrostatic pressure

Disease: Cirrhosis, Familial