Rabbit anti-Human SPHK1 Polyclonal Antibody | anti-SPHK1 antibody

SPHK1 antibody

Cat. Num. AAA535504

• Gene Names: SPHK1; SPHK
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: SPHK1 antibody was purified by affinity chromatography.
• Synonyms: SPHK1; Polyclonal Antibody; SPHK1 antibody; Polyclonal SPHK1; Anti-SPHK1; sphingosine kinase 1; sphingosine kinase type 1; SPHK 1; SPHK -1; SK1; anti-SPHK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human
• Purity/Purification: SPHK1 antibody was purified by affinity chromatography.
• Form/Format: Purified by Immunoaffinity Chromatography and supplied in PBS with 0.1% NaN3.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 398

• Applicable Applications for anti-SPHK1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC-P: 20 ug/ml
• Biological Significance: Sphingosine kinase 1 phosphorylates sphingosine to sphingosine-1-phosphate (S1P) SK1 is normally a cytosolic protein but is recruited to membranes rich in phosphatidate (PA), a product of Phospholipase D (PLD). Various stimuli increase cellular levels of sphingosine-1-phosphate (SPP) by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine.
• Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
• Immunogen: SPHK1 antibody was raised in rabbit using a synthetic peptide conjugated to KLH as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-SPHK1 antibody

Rabbit polyclonal SPHK1 antibody

Product Categories/Family for anti-SPHK1 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 21361088
• NCBI GeneID: 8877
• NCBI Accession #: NP_068807.2
• NCBI GenBank Nucleotide #: NM_021972.3
• UniProt Accession #: Q96GK1; Q8N632; Q9HD92; Q9NY70; Q9NYL3; Q9NYA1
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: sphingosine kinase 1 isoform 1
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Synonym Gene Names: SPHK; SPK; SK 1; SPK 1
• UniProt Entry Name: SPHK1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 2.7.1.91; Kinase, lipid

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: synaptic vesicle; axon; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: calmodulin binding; D-erythro-sphingosine kinase activity; protein binding; DNA binding; protein phosphatase 2A binding; sphinganine kinase activity; magnesium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein folding; positive regulation of NF-kappaB import into nucleus; female pregnancy; positive regulation of mitotic cell cycle; signal transduction; positive regulation of neurotransmitter secretion; activation of NF-kappaB transcription factor; 'de novo' posttranslational protein folding; positive regulation of fibroblast proliferation; response to magnesium ion; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of smooth muscle contraction; inflammatory response; blood vessel development; sphingolipid metabolic process; sphingolipid biosynthetic process; cyclooxygenase pathway; calcium-mediated signaling; sphingosine metabolic process; lipid phosphorylation; cellular response to starvation; positive regulation of cell growth; response to ATP; positive regulation of angiogenesis; cellular protein metabolic process; positive regulation of protein ubiquitination; brain development; positive regulation of protein amino acid phosphorylation; response to progesterone stimulus; vascular endothelial growth factor receptor signaling pathway; sphingoid catabolic process; positive regulation of cell migration; response to amine stimulus; negative regulation of apoptosis