Rabbit Fibronectin Polyclonal Antibody | anti-FN1 antibody
Fibronectin antibody
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
Reactivity
Human fibronectin
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Fibronectin antibody was purified by affinity chromatography.
Synonyms
Fibronectin; Polyclonal Antibody; Fibronectin antibody; Polyclonal Fibronectin; Anti-Fibronectin; Transformation sensitive protein; Migration stimulating factor; Cold insoluble globulin; MSF; LETS; CIG; anti-FN1 antibody
Host
Rabbit
Reactivity
Human fibronectin
Clonality
Polyclonal
Specificity
Human fibronectin
Purity/Purification
Fibronectin antibody was purified by affinity chromatography.
Form/Format
Sterile filtered liquid in 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
49
Applicable Applications for anti-FN1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 1:5,000-1:10,000
IHC: 1:50-1:200
IP: 1:100
WB: 1:5,000-1:10,000
IHC: 1:50-1:200
IP: 1:100
WB: 1:5,000-1:10,000
Biological Significance
Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for anti-FN1 antibody
Affinity purified Rabbit polyclonal Fibronectin antibody
Product Categories/Family for anti-FN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,341 Da
NCBI Official Full Name
fibronectin, partial
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
NCBI Protein Information
fibronectin
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
FN1
UniProt Entry Name
O95608_HUMAN
NCBI Description
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Research Articles on FN1
1. The presence of VN on the material surfaces enhanced cell-mediated FN reorganization and secretion.
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Product Notes
The FN1 fn1 (Catalog #AAA534028) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fibronectin antibody reacts with Human fibronectin and may cross-react with other species as described in the data sheet. AAA Biotech's Fibronectin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:5,000-1:10,000 IHC: 1:50-1:200 IP: 1:100 WB: 1:5,000-1:10,000. Researchers should empirically determine the suitability of the FN1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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