Western Blot (WB)
(Western blot analysis of EGFR expression in MCF7 (A); Raw264.7 (B); PC12 (C) whole cell lysates.)
Rabbit EGFR Polyclonal Antibody | anti-EGFR antibody
EGFR Antibody
Gene Names
Egfr; C-erb; c-erbB; CG10079; D-Egf; d-egf-r; D-EGFR; degfr; dEgfr; dEGFr; dEGFR; Degfr; DEgfr; DEGFR; dEGFR1; der; Der; DER; DER flb; DER/EGFR; DER/top; DER/torpedo; DER1; DmelCG10079; DmHD-33; EC2-4; EFG-R; Egf; Egf-r; EGF-R; egfr; EgfR; EGfr; EGFr; EGFR; EK2
Applications
Western Blot, Immunohistochemistry
Synonyms
EGFR; Polyclonal Antibody; EGFR Antibody; Purified Polyclonal EGFR Antibody; Polyclonal EGFR antibody; Anti-EGFR antibody; ERBB antibody; ERBB1 antibody; HER1 antibody; Epidermal growth factor receptor antibody; Proto-oncogene c-ErbB-1 antibody; Receptor tyrosine-protein kinase erbB-1 antibody; anti-EGFR antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of EGFR protein
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Concentration
1.0mg/mL (varies by lot)
Sequence Length
1426
Applicable Applications for anti-EGFR antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
Immunogen
EGFR antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EGFR
Cross-Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
Preparation and Storage
Upon delivery , aliqout and store at -20°C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of EGFR expression in MCF7 (A); Raw264.7 (B); PC12 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EGFR expression in MCF7 (A); Raw264.7 (B); PC12 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX)
Related Product Information for anti-EGFR antibody
Biological Significance: EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
Product Categories/Family for anti-EGFR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
epidermal growth factor receptor, isoform B
NCBI Official Synonym Full Names
Epidermal growth factor receptor
NCBI Official Symbol
Egfr
NCBI Official Synonym Symbols
C-erb; c-erbB; CG10079; D-Egf; d-egf-r; D-EGFR; degfr; dEgfr; dEGFr; dEGFR; Degfr; DEgfr; DEGFR; dEGFR1; der; Der; DER; DER flb; DER/EGFR; DER/top; DER/torpedo; DER1; DmelCG10079; DmHD-33; EC2-4; EFG-R; Egf; Egf-r; EGF-R; egfr; EgfR; EGfr; EGFr; EGFR; EK2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
Protein Family
UniProt Gene Name
Egfr
UniProt Synonym Gene Names
c-erbB; DER; top; Egfr
Uniprot Description
Binds to four ligands: Spitz, Gurken, Vein and Argos, which is an antagonist. Transduces the signal through the ras-raf-MAPK pathway. Involved in a myriad of developmental decisions. Critical for the proliferation of imaginal tissues, and for the determination of both the antero-posterior and dorso-ventral polarities of the oocyte. In the embryo, plays a role in the establishment of ventral cell fates, maintenance of amnioserosa and ventral neuroectodermal cells, germ band retraction, cell fate specification in the central nervous system and production of cuticle. Required for embryonic epithelial tissue repair.
Research Articles on EGFR
Similar Products
Product Notes
The EGFR egfr (Catalog #AAA5316142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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