Western Blot (WB)
(Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.)
Rabbit Topoisomerase 2 Alpha Polyclonal Antibody | anti-TOP2 antibody
Topoisomerase 2 Alpha Antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Immunogen Affinity Chromatography
Synonyms
Topoisomerase 2 Alpha; Polyclonal Antibody; Topoisomerase 2 Alpha Antibody; Purified Polyclonal Topoisomerase 2 alpha Antibody; Polyclonal Topoisomerase 2 alpha antibody; Anti-Topoisomerase 2 alpha antibody; TOP2 antibody; DNA topoisomerase 2-alpha antibody; DNA topoisomerase II alpha isozyme antibody; anti-TOP2 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Topoisomerase 2 alpha protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
1528
Applicable Applications for anti-TOP2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
IP: 1:10-1:100
IHC: 1:100-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
IP: 1:10-1:100
Immunogen
Topoisomerase 2 alpha antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 alpha as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.)
Related Product Information for anti-TOP2 antibody
Topoisomerase 2 alpha is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication.
Product Categories/Family for anti-TOP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
topoisomerase (DNA) 2 alpha
NCBI Official Synonym Full Names
DNA topoisomerase II alpha
NCBI Official Symbol
Top2a
NCBI Protein Information
DNA topoisomerase 2-alpha
Protein Family
NCBI Description
DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006]
Research Articles on TOP2
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Product Notes
The TOP2 (Catalog #AAA5315120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Topoisomerase 2 Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:100-1:200 IF: 1:100-1:500 ICC: 1:100-1:500 IP: 1:10-1:100. Researchers should empirically determine the suitability of the TOP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase 2 Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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