Western Blot (WB)
(Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser795) antibody)
Rabbit Rb Polyclonal Antibody | anti-RB antibody
Rb Antibody (Ser795)
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
Rb; Polyclonal Antibody; Rb Antibody (Ser795); Rabbit Polyclonal Rb Antibody (Ser795); Polyclonal Rb antibody; Anti-Rb antibody; OSRC antibody; p105-Rb antibody; pp110 antibody; pRb antibody; Rb antibody; RB1 antibody; retinoblastoma 1 antibody; retinoblastoma suspectibility protein antibody; Retinoblastoma-associated protein antibody; anti-RB antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Rb antibody (Ser795) detects endogenous levels of Rb only when phosphorylated at serine 795
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-RB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Rb antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 795 (P-S-SP-P-L) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser795) antibody)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (phospho-Ser795) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb antibody (Ser795))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb antibody (Ser795))
Related Product Information for anti-RB antibody
Rb is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide.
Product Categories/Family for anti-RB antibody
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Product Notes
The RB (Catalog #AAA5313572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the RB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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